Keywords:
Broccoli extract
dexamethasone
NAFLD
hepatic tissue MDA
pancreatic tissue MDA

  • Abstract

    Type 2 diabetes significantly increases the risk of developing nonalcoholic fatty liver disease (NAFLD) due to insulin resistance, resulting in fat accumulation within the liver. Elevated levels of malondialdehyde (MDA) are a reliable indicator of NAFLD. Dexamethasone plays a crucial role in worsening NAFLD by heightening liver inflammation and oxidative stress, which also adversely affects pancreatic cells. This study decisively investigates the impact of dexamethasone-induced diabetes on MDA levels in the liver and pancreas of white rats, while also examining the potent effects of broccoli extract in alleviating this damage. The experiment involved three groups of male rats: a negative control group, a positive control group, and a treatment group. MDA levels were meticulously measured in both liver and pancreatic tissues. The findings unequivocally showed that the treatment group (K3) had the highest MDA levels in the liver, while the negative control group (K1) had the lowest. Remarkably, the rats in group K3, which received broccoli extract at a dosage of 1,000 mg/kg body weight (BW), demonstrated significantly improved MDA levels in liver tissue compared to the positive control group (K2). The differences in liver MDA levels between the control and treatment groups were statistically significant. In terms of pancreatic health, group K2 exhibited the highest MDA levels, while group K1 recorded the lowest, confirming noticeable differences among the groups. Notably, broccoli extract effectively mitigated liver and pancreatic damage resulting from elevated doses of dexamethasone. In conclusion, it is obvious that type 2 diabetes leads to NAFLD through the mechanism of insulin resistance, as indicated by elevated MDA levels. Dexamethasone exacerbates NAFLD by increasing oxidative stress and inflammation. This study compellingly demonstrates that broccoli extract serves as an effective treatment against liver and pancreatic damage caused by dexamethasone.

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How to cite

Mufti, A., Kumala, A. R., Rahayu, I. N., Dagradi, E. M., Husodo, S. D., Dewi, L., Amanda, V. A., & Yuliana, I. S. (2025). Broccoli (<em>Brassica oleracea</em> var. italica) extract empowers the body by lowering MDA levels in the liver and pancreas of male White Wistar rats (<em>Rattus norvegicus</em>) induced by dexamethasone. Multidisciplinary Science Journal, 7(10), 2025503. https://doi.org/10.31893/multiscience.2025503
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