Antibacterial activity of patiwala leaves (<em>Lantana camara</em>) ethanol extract against diabetic ulcers bacteria

Anita Rosanty; Theosobia Grace Orno; Agnes Rantesalu

doi:10.31893/multiscience.2025063

Keywords:

antibacterial activity

Lantana camara

diabetic ulcers

southeast sulawesi

Abstract

Diabetic ulcers are serious complications of diabetes mellitus (DM) affecting several individuals around the world. These complications are caused by impaired blood circulation to peripheral tissue and are further exacerbated by infection due to chronic hyperglycemia, leading to tissue death. Several types of bacteria have been reported to cause infection in diabetic ulcers patients, including Staphylococcus aureus, Staphylococcus epidermis, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Klebsiella pneumoniae, and E. coli. This indicates that there is a pressing need to develop herbal products to minimize complications. Therefore, this study aims to determine antibacterial activity of Patiwala leaves (Lantana camara) ethanol extract against bacteria that cause diabetic ulcers. The identification of bioactive compounds from the ethanol extract of patiwala leaves was carried out qualitatively and antibacterial activity using the Kirby Bauer diffusion method in triplicate. The results showed that Patiwala leaves ethanol extract with a concentration of 100 mg/mL had a strong inhibitory power against Staphylococcus aureus, Staphylococcus epidermis, and Pseudomonas aeruginosa, with values of 18 ± 0.00, 19 ± 0.00 and 18 ± 0.00 (p< 0.05), respectively. Klebsiella pneumonia and E. coli bacteria showed strong potential as inhibitors at a concentration of 200 mg/mL, with an average zone of inhibition of 19 ± 0.00 and 20 ± 0.00 (p < 0.05). Meanwhile, Proteus mirabilis and Proteus vulgaris had moderate inhibitory potential ranging from concentrations of 25-200 mg/mL, with an average of 15 ± 0.00 to 16 ± 2.00 (p < 0.05). Based on these results, Patiwala leaves ethanol extract has the potential to be developed as antibacterial in diabetic ulcers.
References
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How to cite

Rosanty, A., Orno, T. G., & Rantesalu, A. (2024). Antibacterial activity of patiwala leaves (<em>Lantana camara</em>) ethanol extract against diabetic ulcers bacteria. Multidisciplinary Science Journal, 7(2), 2025063. https://doi.org/10.31893/multiscience.2025063

[1] Ahn, J. (2021). Treatment of diabetic foot ulcers. Journal of the Korean Medical Association, 64(8), 529–536. https://doi.org/10.5124/JKMA.2021.64.8.529

[2] Armstrong, D. G., Boulton, A. J. M., & Bus, S. A. (2017). Diabetic foot ulcers and their recurrence. The New England Journal of Medicine, 376(24), 2367–2375. https://doi.org/10.1056/NEJMra1615439

[3] Chen, L., Sun, S., Gao, Y., & Ran, X. (2023). Global mortality of diabetic foot ulcer: A systematic review and meta-analysis of observational studies. Diabetes, Obesity & Metabolism, 25(1), 36–45. https://doi.org/10.1111/dom.14840

[4] CLSI. (2014). Clinical and Laboratory Standards Institute, M100-S24 performance standards for antimicrobial susceptibility testing; 24th informational supplement (Issue January).

[5] Feng, J., Wang, J., Wang, Y., Huang, X., Shao, T., Deng, X., Cao, Y., Zhou, M., & Zhao, C. (2022). Oxidative stress and lipid peroxidation: Prospective associations between ferroptosis and delayed wound healing in diabetic ulcers. Frontiers in Cell and Developmental Biology, 10, 898657. https://doi.org/10.3389/fcell.2022.898657

[6] Hoffstad, O., Mitra, N., Walsh, J., & Margolis, D. J. (2015). Diabetes, lower-extremity amputation, and death. Diabetes Care, 38(10), 1852–1857. https://doi.org/10.2337/dc15-0536

[7] Hussein, L. (2023). Antibacterial activity of Lantana camara flower extracts against growth of pathogenic bacteria isolated from wounds and burns infections. Academic Science Journal, 1, 25–42. https://doi.org/10.24237/ASJ.01.03.638B

[8] Kappala, S., Gorja, A., Mukurala, S., Jabeen, N., & Bandla, J. (2023). Phytochemical screening and in-vitro antibacterial activity evaluation of Lantana camara Linn. leaf extract. Oriental Journal of Chemistry, 39(5), 1321–1324. https://doi.org/10.13005/ojc/390527

[9] Kumar, K., & S J, P. (2019). Antibacterial and antioxidant potential of methanolic leaf extract of Lantana camara. World Journal of Pharmacy and Pharmaceutical Sciences, 8, 622–628. https://doi.org/10.20959/wjpps20199-14571

[10] Lavery, L. A., Ryan, E. C., Ahn, J., Crisologo, P. A., Oz, O. K., La Fontaine, J., & Wukich, D. K. (2020). The infected diabetic foot: Re-evaluating the Infectious Diseases Society of America diabetic foot infection classification. Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America, 70(8), 1573–1579. https://doi.org/10.1093/cid/ciz489

[11] Macdonald, K. E., Boeckh, S., Stacey, H. J., & Jones, J. D. (2021). The microbiology of diabetic foot infections: A meta-analysis. BMC Infectious Diseases, 21(1), 770. https://doi.org/10.1186/s12879-021-06516-7

[12] Meloni, M., Izzo, V., Giurato, L., Lázaro-Martínez, J. L., & Uccioli, L. (2020). Prevalence, clinical aspects and outcomes in a large cohort of persons with diabetic foot disease: Comparison between neuropathic and ischemic ulcers. Journal of Clinical Medicine, 9(6). https://doi.org/10.3390/jcm9061780

[13] Mz, K., Suryati, S., & Efdi, M. (2018). A triterpenoid compound from the leaves of Lantana camara Linn. Indonesian Journal of Fundamental and Applied Chemistry, 3, 18–22. https://doi.org/10.24845/ijfac.v3.i1.18

[14] Nath, A., Shibli, M., & Arumugam, S. (2021). Anti-hyperglycemic and anti-oxidant activities of ethanolic extract of Lantana camara leaves. International Journal of Frontiers in Life Science Research, 1, 5–15. https://doi.org/10.53294/ijflsr.2021.1.2.0043

[15] Oladoye, S., Falade, V., Adewusi John, A., & Ibikunle, G. (2021). Lantana camara: Phyto-constituents and antimicrobial activity study. Pan African Journal of Life Sciences, 5. https://doi.org/10.36108/pajols/1202.50.0270

[16] Orno, T., & Rantesalu, A. (2020). In vitro cytotoxicity assays of seagrass (Enhalus acoroides) methanol extract from Soropia coastal waters Southeast Sulawesi regency. Indonesian Journal of Medical Laboratory Science and Technology, 2, 27–33. https://doi.org/10.33086/ijmlst.v2i1.1463

[17] P., - F. K., - S. A., - G. U. K., & - A. K. (2023). A comparative study: In-vitro anti-microbial activity of various parts of Lantana Camara using ethanolic extract. International Journal for Multidisciplinary Research. https://api.semanticscholar.org/CorpusID:257448663

[18] Pany, S., Sen, S., Prasanna, G., Pati, S., & Pal, B. (2021). Spectrum of bacterial infections associated with diabetic ulcer patients. Journal of Pure and Applied Microbiology, 15. https://doi.org/10.22207/JPAM.15.2.01

[19] Patil, A., More, D., Patil, A., Jadhav, K. A., Vijil Mejia, M. E., & Patil, S. S. (2018). Clinical, etiological, anatomical, and bacteriological study of “diabetic foot” patients: Results of a single center study. Cureus, 10(4), e2498. https://doi.org/10.7759/cureus.2498

[20] Saeedi, P., Petersohn, I., Salpea, P., Malanda, B., Karuranga, S., Unwin, N., Colagiuri, S., Guariguata, L., Motala, A. A., Ogurtsova, K., Shaw, J. E., Bright, D., & Williams, R. (2019). Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the International Diabetes Federation Diabetes Atlas, 9th edition. Diabetes Research and Clinical Practice, 157, 107843. https://doi.org/10.1016/j.diabres.2019.107843

[21] Sannathimmappa, M. B., Nambiar, V., Aravindakshan, R., Al Khabori, M. S. J., Al-Flaiti, A. H. S., Al-Azri, K. N. M., Al-Reesi, A. K. S., & Al Kiyumi, A. R. M. (2021). Diabetic foot infections: Profile and antibiotic susceptibility patterns of bacterial isolates in a tertiary care hospital of Oman. Journal of Education and Health Promotion, 10, 254. https://doi.org/10.4103/jehp.jehp_1552_20

[22] Syauta, D., Mulawardi, Prihantono, Hendarto, J., Mariana, N., Sulmiati, Kusumanegara, J., & Faruk, M. (2021). Risk factors affecting the degree of diabetic foot ulcers according to Wagner classification in diabetic foot patients. Medicina Clínica Práctica, 4, 100231. https://doi.org/10.1016/j.mcpsp.2021.100231

[23] Tamboli, F. A., Thorat, V. H., Jadhav, A. S., & Chavan, R. (2022). Phytochemical analysis and antimicrobial activity of Lantana camara. International Journal of Pharmaceutical Chemistry and Analysis, 8(4), 171–173. https://doi.org/10.18231/j.ijpca.2021.033

[24] Tim Riskesda. (2018). Laporan Riskesdas 2018 Nasional.pdf. In Lembaga Penerbit Balitbangkes (p. hal 156). https://repository.badankebijakan.kemkes.go.id/id/eprint/3514/1/Laporan%20Riskesdas%202018%20Nasional.pdf

[25] World Health Organization. (2016). Global report on diabetes. Isbn, 978, 88. https://doi.org/ISBN%20978%2092%204%20156525%207

[26] World Health Organization. (2021). Diabetes. Diabetes - Fact Sheet. https://www.who.int/newsroom/fact-sheets/detail/diabetes

Multidisciplinary Science Journal

Research Article | Vol. 7 Issue 2 (2025), e2025063 | Published in 05 August 2024

Antibacterial activity of patiwala leaves (Lantana camara) ethanol extract against diabetic ulcers bacteria

Abstract

References

How to cite