Fabrication, characterization and toxicity analysis of erbium doped hydroxyapatite

Tahoora Taskeen L; Taanya Imtiaz; Nidhita Suresh; Saranya K

Keywords:

Abstract

Introduction

The use of bioactive inorganic materials in many clinical applications has garnered considerable attention. The demand for synthetic biomaterials to restore or/and replace bone tissue lost to injury or disease, which has significantly expanded in recent years, serves as an excellent illustration of the necessity for such materials.Erbium-doped hydroxyapatite (Er-HAp) is a promising material with potential applications in biomedical and optical fields due to its unique properties. It has been a new source to develop tissue engineering using erbium in addition to depict bioluminescence and biomineralization properties.

Materials and methods:

Erbium doped hydroxyapatite was synthesized and subjected to EDX, XRD, ATR-IR and SEM analysis for analyzing the structural and mechanical properties of the synthesized novel complex. The toxicity analysis of the complex was done with zebra fish.

Results:

The formed complex confirmed an in-depth analysis of the crystals formed were further studied using ATR-IR and XRD analysis. In the zebrafish toxicity analysis, hydroxyapatite doped with erbium did not exhibit any toxicological effects.

Conclusion:

Erbium doped hydroxyapatite can be used as an efficient bone grafting biomaterial for bone regeneration. Future studies would be done invitro and invivo to assess its efficacy in bioactivity when used as a biomaterial for guided bone regeneration.
References
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3. Zhang Y, Jo JI, Chen L, Hontsu S, Hashimoto Y. Effect of Hydroxyapatite Coating by Er: YAG Pulsed Laser Deposition on the Bone Formation Efficacy by Polycaprolactone Porous Scaffold. Int J Mol Sci [Internet]. 2022 Aug 12;23(16). Available from: http://dx.doi.org/10.3390/ijms23169048
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5. Ceballos-Jiménez AY, Rodríguez-Vilchis LE, Contreras-Bulnes R, Alatorre JÁA, Velazquez-Enriquez U, García-Fabila MM. Acid resistance of dental enamel treated with remineralizing agents, Er:YAG laser and combined treatments. Dent Med Probl. 2018 Jul-Sep;55(3):255–9.
6. Ahmed LO, Bulut N, Kebiroglu H, Alkhedher M, Ates T, Koytepe S, et al. Effects of Yttrium Doping on Erbium-Based Hydroxyapatites: Theoretical and Experimental Study. Materials [Internet]. 2022 Oct 16;15(20). Available from: http://dx.doi.org/10.3390/ma15207211
7. Kurt S, Kırtıloğlu T, Yılmaz NA, Ertaş E, Oruçoğlu H. Evaluation of the effects of Er:YAG laser, Nd:YAG laser, and two different desensitizers on dentin permeability: in vitro study. Lasers Med Sci. 2018 Dec;33(9):1883–90.
8. Kumar G, Rehman F, Chaturvedy V. Soft Tissue Applications of Er,Cr:YSGG Laser in Pediatric Dentistry. Int J Clin Pediatr Dent. 2017 Jun 1;10(2):188–92.
9. Thirumalai J. Hydroxyapatite: Advances in Composite Nanomaterials, Biomedical Applications and Its Technological Facets. BoD – Books on Demand; 2018. 188 p.
10. Tithito T, Sillapaprayoon S, Pimtong W, Thongbunchoo J, Charoenphandhu N, Krishnamra N, et al. Development of Biomaterials Based on Biomimetic Trace Elements Co-Doped Hydroxyapatite: Physical, In Vitro Osteoblast-like Cell Growth and In Vivo Cytotoxicity in Zebrafish Studies. Nanomaterials (Basel) [Internet]. 2023 Jan 6;13(2). Available from: http://dx.doi.org/10.3390/nano13020255
11. Gao M, Yang Y, Lv M, Song W, Song Z. Oxidative stress and DNA damage in zebrafish liver due to hydroxyapatite nanoparticles-loaded cadmium. Chemosphere. 2018 Jul;202:498–505.
12. Patidar AP, Patel MB, Valand NN. Metal Complexes as an Artificial Metallonucleases: An Overview of Synthesis, Characterization and Biological Interphase of Some Copper (II) and Palladium (II) Complexes. LAP Lambert Academic Publishing; 2015. 108 p.
13. Khan AF, Saleem M, Afzal A, Ali A, Khan A, Khan AR. Bioactive behavior of silicon substituted calcium phosphate based bioceramics for bone regeneration. Mater Sci Eng C Mater Biol Appl. 2014 Feb 1;35:245–52.
14. Čeh M, Gec M. SEM and EDX analysis of polished PMN-PT single crystal and polycrystalline PMN-PT matrix. 2005.
15. Rehan K, Rehan I, Sultana S, Khan F. Spectrochemical Analysis of Nutritional and Toxic Metals in Different Brands of Candies Using Advanced Diagnostic Approaches. Biol Trace Elem Res [Internet]. 2023 Nov 7; Available from: http://dx.doi.org/10.1007/s12011-023-03945-0
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18. Riaz M, Zia R, Ijaz A, Hussain T, Mohsin M, Malik A. Synthesis of monophasic Ag doped hydroxyapatite and evaluation of antibacterial activity. Mater Sci Eng C Mater Biol Appl. 2018 Sep 1;90:308–13.
19. Petrone L, McQuillan J. Adhesives from Marine Microorganisms: Characterization and ATR-IR Adsorption Studies of Related Model Compounds. 2013. 200 p.
20. Lumpi D, Braunshier C. Effective Reaction Monitoring of Intermediates by ATR-IR Spectroscopy Utilizing Fibre Optic Probes. 2012.
21. Mondal S, Van Tu N, Park S, Choi Jaeyeop, Le Hai T, Yi Myunggi et al. Bioactive, luminescent erbium-doped hydroxyapatite nanocrystals for biomedical applications, Ceramics International. Part B. 2020;46(10):16020-31, ISSN 0272-8842. doi: 10.1016/j.ceramint.2020.03.152.
22. de Souza AM, Araujo-Silva H, Costa AM, Rossi AL, Rossi AM, Granjeiro JM, et al. Embryotoxicity and visual-motor response of functionalized nanostructured hydroxyapatite-based biomaterials in zebrafish (Danio rerio). Chemosphere. 2023 Feb;313:137519.
23. Meenakshi SS, Sankari M. Effectiveness of Chitosan Nanohydrogel as a Bone Regenerative Material in Intrabony Defects in Patients With Chronic Periodontitis: A Randomized Clinical Trial. Journal of Advanced Oral Research. 2021;12(2):222-228. doi:10.1177/2320206821998574
24. Karunakaran G, Cho EB, Kumar GS, Kolesnikov E, Govindaraj SK, Mariyappan K, et al. CTAB enabled microwave-hydrothermal assisted mesoporous Zn-doped hydroxyapatite nanorods synthesis using bio-waste Nodipecten nodosus scallop for biomedical implant applications. Environ Res. 2023 Jan 1;216(Pt 3):114683.
25. Garapati B, Malaiappan S, Rajeshkumar S, Murthykumar K. Cytotoxicity of lycopene-mediated silver nanoparticles in the embryonic development of zebrafish-An animal study. J Biochem Mol Toxicol. 2022 Oct;36(10):e23173. doi: 10.1002/jbt.23173. Epub 2022 Jul 13. PMID: 35822638.
26. Tomić SL, Nikodinović-Runić J, Vukomanović M, Babić MM, Vuković JS. Novel Hydrogel Scaffolds Based on Alginate, Gelatin, 2-Hydroxyethyl Methacrylate, and Hydroxyapatite. Polymers [Internet]. 2021 Mar 18;13(6). Available from: http://dx.doi.org/10.3390/polym13060932

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to cite

Taskeen L , T., Imtiaz, T., Suresh, N., & K, S. (2024). Fabrication, characterization and toxicity analysis of erbium doped hydroxyapatite. Multidisciplinary Science Journal, (| Accepted Articles). Retrieved from https://malque.pub/ojs/index.php/msj/article/view/2136

[1] Culp TD. Photoluminescence Properties of Erbium Doped III-V Semiconductors. 1998. 556 p.

[2] Neacsu IA, Stoica AE, Vasile BS, Andronescu E. Luminescent Hydroxyapatite Doped with Rare Earth Elements for Biomedical Applications. Nanomaterials (Basel) [Internet]. 2019 Feb 10;9(2). Available from: http://dx.doi.org/10.3390/nano9020239

[3] Zhang Y, Jo JI, Chen L, Hontsu S, Hashimoto Y. Effect of Hydroxyapatite Coating by Er: YAG Pulsed Laser Deposition on the Bone Formation Efficacy by Polycaprolactone Porous Scaffold. Int J Mol Sci [Internet]. 2022 Aug 12;23(16). Available from: http://dx.doi.org/10.3390/ijms23169048

[4] Chen L, Hontsu S, Komasa S, Yamamoto E, Hashimoto Y, Matsumoto N. Hydroxyapatite Film Coating by Er:YAG Pulsed Laser Deposition Method for the Repair of Enamel Defects. Materials [Internet]. 2021 Dec 6;14(23). Available from: http://dx.doi.org/10.3390/ma14237475

[5] Ceballos-Jiménez AY, Rodríguez-Vilchis LE, Contreras-Bulnes R, Alatorre JÁA, Velazquez-Enriquez U, García-Fabila MM. Acid resistance of dental enamel treated with remineralizing agents, Er:YAG laser and combined treatments. Dent Med Probl. 2018 Jul-Sep;55(3):255–9.

[6] Ahmed LO, Bulut N, Kebiroglu H, Alkhedher M, Ates T, Koytepe S, et al. Effects of Yttrium Doping on Erbium-Based Hydroxyapatites: Theoretical and Experimental Study. Materials [Internet]. 2022 Oct 16;15(20). Available from: http://dx.doi.org/10.3390/ma15207211

[7] Kurt S, Kırtıloğlu T, Yılmaz NA, Ertaş E, Oruçoğlu H. Evaluation of the effects of Er:YAG laser, Nd:YAG laser, and two different desensitizers on dentin permeability: in vitro study. Lasers Med Sci. 2018 Dec;33(9):1883–90.

[8] Kumar G, Rehman F, Chaturvedy V. Soft Tissue Applications of Er,Cr:YSGG Laser in Pediatric Dentistry. Int J Clin Pediatr Dent. 2017 Jun 1;10(2):188–92.

[9] Thirumalai J. Hydroxyapatite: Advances in Composite Nanomaterials, Biomedical Applications and Its Technological Facets. BoD – Books on Demand; 2018. 188 p.

[10] Tithito T, Sillapaprayoon S, Pimtong W, Thongbunchoo J, Charoenphandhu N, Krishnamra N, et al. Development of Biomaterials Based on Biomimetic Trace Elements Co-Doped Hydroxyapatite: Physical, In Vitro Osteoblast-like Cell Growth and In Vivo Cytotoxicity in Zebrafish Studies. Nanomaterials (Basel) [Internet]. 2023 Jan 6;13(2). Available from: http://dx.doi.org/10.3390/nano13020255

[11] Gao M, Yang Y, Lv M, Song W, Song Z. Oxidative stress and DNA damage in zebrafish liver due to hydroxyapatite nanoparticles-loaded cadmium. Chemosphere. 2018 Jul;202:498–505.

[12] Patidar AP, Patel MB, Valand NN. Metal Complexes as an Artificial Metallonucleases: An Overview of Synthesis, Characterization and Biological Interphase of Some Copper (II) and Palladium (II) Complexes. LAP Lambert Academic Publishing; 2015. 108 p.

[13] Khan AF, Saleem M, Afzal A, Ali A, Khan A, Khan AR. Bioactive behavior of silicon substituted calcium phosphate based bioceramics for bone regeneration. Mater Sci Eng C Mater Biol Appl. 2014 Feb 1;35:245–52.

[14] Čeh M, Gec M. SEM and EDX analysis of polished PMN-PT single crystal and polycrystalline PMN-PT matrix. 2005.

[15] Rehan K, Rehan I, Sultana S, Khan F. Spectrochemical Analysis of Nutritional and Toxic Metals in Different Brands of Candies Using Advanced Diagnostic Approaches. Biol Trace Elem Res [Internet]. 2023 Nov 7; Available from: http://dx.doi.org/10.1007/s12011-023-03945-0

[16] Minh DP. Design and Applications of Hydroxyapatite-Based Catalysts. John Wiley & Sons; 2022. 580 p.

[17] Mucalo M. Hydroxyapatite (HAp) for Biomedical Applications. Elsevier; 2015. 404 p.

[18] Riaz M, Zia R, Ijaz A, Hussain T, Mohsin M, Malik A. Synthesis of monophasic Ag doped hydroxyapatite and evaluation of antibacterial activity. Mater Sci Eng C Mater Biol Appl. 2018 Sep 1;90:308–13.

[19] Petrone L, McQuillan J. Adhesives from Marine Microorganisms: Characterization and ATR-IR Adsorption Studies of Related Model Compounds. 2013. 200 p.

[20] Lumpi D, Braunshier C. Effective Reaction Monitoring of Intermediates by ATR-IR Spectroscopy Utilizing Fibre Optic Probes. 2012.

[21] Mondal S, Van Tu N, Park S, Choi Jaeyeop, Le Hai T, Yi Myunggi et al. Bioactive, luminescent erbium-doped hydroxyapatite nanocrystals for biomedical applications, Ceramics International. Part B. 2020;46(10):16020-31, ISSN 0272-8842. doi: 10.1016/j.ceramint.2020.03.152.

[22] de Souza AM, Araujo-Silva H, Costa AM, Rossi AL, Rossi AM, Granjeiro JM, et al. Embryotoxicity and visual-motor response of functionalized nanostructured hydroxyapatite-based biomaterials in zebrafish (Danio rerio). Chemosphere. 2023 Feb;313:137519.

[23] Meenakshi SS, Sankari M. Effectiveness of Chitosan Nanohydrogel as a Bone Regenerative Material in Intrabony Defects in Patients With Chronic Periodontitis: A Randomized Clinical Trial. Journal of Advanced Oral Research. 2021;12(2):222-228. doi:10.1177/2320206821998574

[24] Karunakaran G, Cho EB, Kumar GS, Kolesnikov E, Govindaraj SK, Mariyappan K, et al. CTAB enabled microwave-hydrothermal assisted mesoporous Zn-doped hydroxyapatite nanorods synthesis using bio-waste Nodipecten nodosus scallop for biomedical implant applications. Environ Res. 2023 Jan 1;216(Pt 3):114683.

[25] Garapati B, Malaiappan S, Rajeshkumar S, Murthykumar K. Cytotoxicity of lycopene-mediated silver nanoparticles in the embryonic development of zebrafish-An animal study. J Biochem Mol Toxicol. 2022 Oct;36(10):e23173. doi: 10.1002/jbt.23173. Epub 2022 Jul 13. PMID: 35822638.

[26] Tomić SL, Nikodinović-Runić J, Vukomanović M, Babić MM, Vuković JS. Novel Hydrogel Scaffolds Based on Alginate, Gelatin, 2-Hydroxyethyl Methacrylate, and Hydroxyapatite. Polymers [Internet]. 2021 Mar 18;13(6). Available from: http://dx.doi.org/10.3390/polym13060932

Abstract

References

How to cite