Neutrophils of diploid and triploid rainbow trouts assessed by flow cytometry


  • Renata Stecca Iunes University of São Paulo, São Paulo, SP, Brazil
  • André Luiz Veiga Conrado University of São Paulo, São Paulo, SP, Brazil
  • José Roberto Kfoury Junior University of São Paulo, São Paulo, SP, Brazil



leucocytes, monoclonal antibody, Oncorhynchus mykiss, triploid fish


Triploid rainbow trouts Oncorhynchus mykiss are reared in Brazil, but there is no data about haematological differences when compared to diploid ones. Due to the lack of information, the goal was to evaluate the neutrophils of adult diploid and triploid rainbow trouts O. mykiss by flow cytometry screening using a monoclonal anti-neutrophil antibody (TTL-5E9). In comparison, the population of neutrophils was proportionally larger in diploid trouts (9.94 ± 4.64% vs. 5.58 ± 1.09, p = 0.0481), but with cells showing smaller size and less complexity than the neutrophils of triploid trouts. As sorted neutrophils were not evaluated on phagocytic activity or apoptosis induction, it was not possible to establish neutrophil activity differences between ploidies.


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Population profile of the leukocyte of triploid trout



PUBLISHED: 2021-12-01

How to Cite

Iunes, R. S., Conrado, A. L. V., & Kfoury Junior, J. R. (2021). Neutrophils of diploid and triploid rainbow trouts assessed by flow cytometry. Multidisciplinary Science Journal, 4(1), e2022003.



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