A brief review on recent advances in reverse phase HPLC

Swati Pund; Sapnarani Nalawade; Vikas Rajurkar; Suresh Jayatpal; Nitin Deshmukh; Harshal Tare

doi:10.31893/multirev.2024072

Keywords:

Abstract

Modern chromatography is mostly used for analytical purposes. Modern pharmaceutical and biological analysis uses HPLC. It separates molecules from heterogeneous liquids and characterizes them. Mass spectrometry identifies peptides, proteins, and other compounds after high-performance liquid chromatography. HPLC's flexibility and dependability (pressure-driven liquid support) make it popular. However, fast separation often creates high operating pressure, which stresses HPLC equipment. Thus, core-shell silica microspheres and zirconia packing have been produced recently. Fused-core or superficially porous microspheres have solid cores with porous shells. Despite its low pressure, this device can separate small, large, and intricate molecules. It is effective and fast. UPLC offers greater resolution, sensitivity, and separation times than HPLC; however, it may be less reproducible. Rapid Resolution Liquid Chromatography (RRLC) is more precise and sensitive than HPLC, improving sample analysis quality and quantity. This review will highlight column technology basics and RP-HPLC advances and focuses on the concept of HPLC.
References
1. Ahmad S, Ganbase M, Deshmukh N, Patil K, Barde L, Deshpande M, Tajane P (2023) Development of validated HPTLC and UHPLC methods for quantification of withanolide in extract and formulation. International Journal of Experimental Research and Reviews 30: 375-387.https://doi.org/10.52756/ijerr.2023.v30.035
2. Ahmad S, Mujawar T, Batewal B, More P, Gaikwad A, Chumbhale D, Tare H (2023) RP-UHPLC and HPTLC method development and validation for analysis of andrographolide from herbal hepatoprotective formulation. International Journal of Pharmaceutical Quality Assurance 14:96-104.
3. Ali SNS, Mobina L, Mehfuza M, Seema P, Ahmed A, Khan GJ (2021) Analytical method development and validation and forced degradation stability-indicating studies of favipiravir by RP-HPLC and UV in bulk and pharmaceutical dosage form. Journal of Pharmaceutical Research International 33:254-271. https://doi.org/10.9734/JPRI/2021/v33i48B33283
4. Bawa RA, Addotey JN, Brobbey AA, Oppong-Kyekyeku J, Hlordzi CK (2023) Bioassay and RP-HPLC method development for the analysis of extracts and herbal products containing Anthocleista nobilis. International Journal of Analytical Chemistry : 2023. https://doi.org/10.1155/2023/6957519
5. Bhatkar TV, Badkhal AV, Bhajipale NS (2020) Stability indicating RP-HPLC method development and validation for the estimation of remogliflozin etabonate in bulk and pharmaceutical dosage form. International Journal of Pharmaceutical Research 12:160-169.
6. Chaudhari VS, Borkar RM, Murty US, Banerjee S (2020) Analytical method development and validation of reverse-phase high-performance liquid chromatography (RP-HPLC) method for simultaneous quantifications of quercetin and piperine in dual-drug loaded nanostructured lipid carriers. Journal of Pharmaceutical and Biomedical Analysis 186: 113325. https://doi.org/10.1016/j.jpba.2020.113325
7. Digge VG, Patil SS, Panchal CV (2022) Stability indicating method development and validation for valacyclovir in bulk and tablet dosage form by RP-HPLC. Journal of Complementary Medicine Research 12(4):30.
8. http://dx.doi.org/10.5455/jcmr.2021.12.04.05
9. Gopireddy RR, Maruthapillai A, Devikala S, Tamilselvi M, Selvi JA, Mahapatra S (2019) DOE approach: a validated stability indicating RP-HPLC method development for the separation of diasteromeric analogs and process impurities of carfilzomib. Materials Today: Proceedings 14:514-531.
10. https://doi.org/10.1016/j.matpr.2019.04.174
11. Habash IW, Al-Shdefat RI, Hailat MM, Dayyih WA (2020) A stability indicating RP-HPLC method development for simultaneous estimation of alogliptin, pioglitazone, and metformin in pharmaceutical formulations. Acta Poloniae Pharmaceutica-Drug Research 77:549-562.
12. https://doi.org/10.32383/appdr/125774
13. Hebbar S, Dubey A, Ravi GS, Kumar H, Saha S (2019) RP-HPLC method development and validation of asiatic acid isolated from the plant Centella asiatica. International Journal of Applied Pharmaceutics 11:72-78 https://doi.org/10.22159/ijap.2019v11i3.31525
14. Itagimatha N, Manjunatha DH (2019, July) RP-HPLC-UV method development and validation for simultaneous determination of terbutaline sulphate, ambroxol HCl and guaifenesin in pure and dosage forms. In Annales pharmaceutiques françaises 77:295-301. https://doi.org/10.1016/j.pharma.2019.02.004
15. Khandare B, Musle AC, Arole SS, Popalghat PV (2019) Analytical method development and validation of olmutinib bulk drug as per ICH Q2 guidelines by using RP-HPLC method. Journal of Drug Delivery and Therapeutics 9:608-611. https://doi.org/10.22270/jddt.v9i4-A.3527
16. Kumar D, Kumar A, Kumar V, Raj A, Rai RR, Baliyan V, Kumar N (2023) A comprehensive review on analytical method development using rp-hplc and recent advances in pharmaceutical applications. Journal for Research in Applied Sciences and Biotechnology 2:53-60. https://doi.org/10.55544/jrasb.2.2.9
17. Kumar GR, Babu BS, Rao RR, Vardhan VM, Abbaraju VDN (2021) Method development and validation of a specific stability indicating RP-HPLC method for molnupiravir API. Journal of Pharmaceutical Research International, 33(60B), 3026-3035.
18. Mahrouse MA, Lamie NT (2019) Experimental design methodology for optimization and robustness determination in ion pair RP-HPLC method development: Application for the simultaneous determination of metformin hydrochloride, alogliptin benzoate and repaglinide in tablets. Microchemical Journal 147:691-706. https://doi.org/10.1016/j.microc.2019.03.038
19. Majumder MRH, Roy P, Pramanik R, Hasan MN (2019) Method development and validation of RP-HPLC method for estimation of luliconazole in marketed formulation (cream). Pharma Innov. J 85:103-108.
20. Mangla B, Beg S, Alam O, Ahsan W, Haque A, Patel KS, Kohli K (2020) Systematic development and validation of RP-HPLC method for simultaneous estimation of tamoxifen and sulphoraphane with specific application for nanolipidic formulations. Arabian Journal of Chemistry 13(11): 7909-7920.
21. Manoranjani M (2021) A study of method development, validation and forced degradation for simultaneous quantification of cisplatin and fluorouracil in bulk and pharmaceutical dosage form by RP-HPLC. Journal of Pharmaceutical Sciences and Research 13:155-61.https://doi.org/10.1016/j.arabjc.2020.09.022
22. Mishra K, Behera S, Mishra SR, Somesu M, & Kiran Kumar B (2019) A validated stability indicating RP-HPLC method development for platelet aggregation inhibitor ticagrelor in bulk and tablet formulation. J Glob Pharma Tech 11:12-18.
23. Subramanian VB, Katari NK, Dongala T, Jonnalagadda S B (2020) Stability‐indicating RP‐HPLC method development and validation for determination of nine impurities in apixaban tablet dosage forms robustness study by quality by design approach. Biomedical Chromatography 34:e4719. https://doi.org/10.1002/bmc.4719

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How to cite

Pund, S., Nalawade, S., Rajurkar, V., Jayatpal, S., Deshmukh, N., & Tare, H. (2024). A brief review on recent advances in reverse phase HPLC. Multidisciplinary Reviews, 7(4), 2024072. https://doi.org/10.31893/multirev.2024072

[1] Ahmad S, Ganbase M, Deshmukh N, Patil K, Barde L, Deshpande M, Tajane P (2023) Development of validated HPTLC and UHPLC methods for quantification of withanolide in extract and formulation. International Journal of Experimental Research and Reviews 30: 375-387.https://doi.org/10.52756/ijerr.2023.v30.035

[2] Ahmad S, Mujawar T, Batewal B, More P, Gaikwad A, Chumbhale D, Tare H (2023) RP-UHPLC and HPTLC method development and validation for analysis of andrographolide from herbal hepatoprotective formulation. International Journal of Pharmaceutical Quality Assurance 14:96-104.

[3] Ali SNS, Mobina L, Mehfuza M, Seema P, Ahmed A, Khan GJ (2021) Analytical method development and validation and forced degradation stability-indicating studies of favipiravir by RP-HPLC and UV in bulk and pharmaceutical dosage form. Journal of Pharmaceutical Research International 33:254-271. https://doi.org/10.9734/JPRI/2021/v33i48B33283

[4] Bawa RA, Addotey JN, Brobbey AA, Oppong-Kyekyeku J, Hlordzi CK (2023) Bioassay and RP-HPLC method development for the analysis of extracts and herbal products containing Anthocleista nobilis. International Journal of Analytical Chemistry : 2023. https://doi.org/10.1155/2023/6957519

[5] Bhatkar TV, Badkhal AV, Bhajipale NS (2020) Stability indicating RP-HPLC method development and validation for the estimation of remogliflozin etabonate in bulk and pharmaceutical dosage form. International Journal of Pharmaceutical Research 12:160-169.

[6] Chaudhari VS, Borkar RM, Murty US, Banerjee S (2020) Analytical method development and validation of reverse-phase high-performance liquid chromatography (RP-HPLC) method for simultaneous quantifications of quercetin and piperine in dual-drug loaded nanostructured lipid carriers. Journal of Pharmaceutical and Biomedical Analysis 186: 113325. https://doi.org/10.1016/j.jpba.2020.113325

[7] Digge VG, Patil SS, Panchal CV (2022) Stability indicating method development and validation for valacyclovir in bulk and tablet dosage form by RP-HPLC. Journal of Complementary Medicine Research 12(4):30.

[8] http://dx.doi.org/10.5455/jcmr.2021.12.04.05

[9] Gopireddy RR, Maruthapillai A, Devikala S, Tamilselvi M, Selvi JA, Mahapatra S (2019) DOE approach: a validated stability indicating RP-HPLC method development for the separation of diasteromeric analogs and process impurities of carfilzomib. Materials Today: Proceedings 14:514-531.

[10] https://doi.org/10.1016/j.matpr.2019.04.174

[11] Habash IW, Al-Shdefat RI, Hailat MM, Dayyih WA (2020) A stability indicating RP-HPLC method development for simultaneous estimation of alogliptin, pioglitazone, and metformin in pharmaceutical formulations. Acta Poloniae Pharmaceutica-Drug Research 77:549-562.

[12] https://doi.org/10.32383/appdr/125774

[13] Hebbar S, Dubey A, Ravi GS, Kumar H, Saha S (2019) RP-HPLC method development and validation of asiatic acid isolated from the plant Centella asiatica. International Journal of Applied Pharmaceutics 11:72-78 https://doi.org/10.22159/ijap.2019v11i3.31525

[14] Itagimatha N, Manjunatha DH (2019, July) RP-HPLC-UV method development and validation for simultaneous determination of terbutaline sulphate, ambroxol HCl and guaifenesin in pure and dosage forms. In Annales pharmaceutiques françaises 77:295-301. https://doi.org/10.1016/j.pharma.2019.02.004

[15] Khandare B, Musle AC, Arole SS, Popalghat PV (2019) Analytical method development and validation of olmutinib bulk drug as per ICH Q2 guidelines by using RP-HPLC method. Journal of Drug Delivery and Therapeutics 9:608-611. https://doi.org/10.22270/jddt.v9i4-A.3527

[16] Kumar D, Kumar A, Kumar V, Raj A, Rai RR, Baliyan V, Kumar N (2023) A comprehensive review on analytical method development using rp-hplc and recent advances in pharmaceutical applications. Journal for Research in Applied Sciences and Biotechnology 2:53-60. https://doi.org/10.55544/jrasb.2.2.9

[17] Kumar GR, Babu BS, Rao RR, Vardhan VM, Abbaraju VDN (2021) Method development and validation of a specific stability indicating RP-HPLC method for molnupiravir API. Journal of Pharmaceutical Research International, 33(60B), 3026-3035.

[18] Mahrouse MA, Lamie NT (2019) Experimental design methodology for optimization and robustness determination in ion pair RP-HPLC method development: Application for the simultaneous determination of metformin hydrochloride, alogliptin benzoate and repaglinide in tablets. Microchemical Journal 147:691-706. https://doi.org/10.1016/j.microc.2019.03.038

[19] Majumder MRH, Roy P, Pramanik R, Hasan MN (2019) Method development and validation of RP-HPLC method for estimation of luliconazole in marketed formulation (cream). Pharma Innov. J 85:103-108.

[20] Mangla B, Beg S, Alam O, Ahsan W, Haque A, Patel KS, Kohli K (2020) Systematic development and validation of RP-HPLC method for simultaneous estimation of tamoxifen and sulphoraphane with specific application for nanolipidic formulations. Arabian Journal of Chemistry 13(11): 7909-7920.

[21] Manoranjani M (2021) A study of method development, validation and forced degradation for simultaneous quantification of cisplatin and fluorouracil in bulk and pharmaceutical dosage form by RP-HPLC. Journal of Pharmaceutical Sciences and Research 13:155-61.https://doi.org/10.1016/j.arabjc.2020.09.022

[22] Mishra K, Behera S, Mishra SR, Somesu M, & Kiran Kumar B (2019) A validated stability indicating RP-HPLC method development for platelet aggregation inhibitor ticagrelor in bulk and tablet formulation. J Glob Pharma Tech 11:12-18.

[23] Subramanian VB, Katari NK, Dongala T, Jonnalagadda S B (2020) Stability‐indicating RP‐HPLC method development and validation for determination of nine impurities in apixaban tablet dosage forms robustness study by quality by design approach. Biomedical Chromatography 34:e4719. https://doi.org/10.1002/bmc.4719

Abstract

References

How to cite