The protective effect of wild mustard (Sinapis arvensis L.) pollen seeds against the toxicity of a solvent (EGME) in Wistar rats

Authors

  • Leila Hamdi Animal Ecophysiology Laboratory, Department of Biology, Faculty of Sciences; Badji Mokhtar Annaba University Algeria.
  • Samir Djemli Applied Neuroendocrinology Laboratory, Department of Biology, Faculty of Sciences, Badji Mokhtar Annaba University Algeria. https://orcid.org/0000-0003-4240-0179
  • Fatma Zohra Arkoub Animal Ecophysiology Laboratory, Department of Biology, Faculty of Sciences; Badji Mokhtar Annaba University Algeria.
  • Rahma Boukarine Animal Ecophysiology Laboratory, Department of Biology, Faculty of Sciences; Badji Mokhtar Annaba University Algeria.
  • Kamel Khelili Animal Ecophysiology Laboratory, Department of Biology, Faculty of Sciences; Badji Mokhtar Annaba University Algeria.
  • Cherif Abdennour Animal Ecophysiology Laboratory, Department of Biology, Faculty of Sciences; Badji Mokhtar Annaba University Algeria.
  • Abdelkrim Tahraoui Applied Neuroendocrinology Laboratory, Department of Biology, Faculty of Sciences, Badji Mokhtar Annaba University Algeria. https://orcid.org/0000-0002-4855-4112

Keywords:

environmental pollution, ethylene glycol monoethyl ether, hematotoxicity, pollen, reprotoxicity, wild mustard

Abstract

Environmental pollution is the unfavorable alteration of our environment, wholly or mainly as a by-product of human actions, by the direct or indirect effects of changes in energy patterns, radiation levels, the chemical and physical constitution, and the abundance of the organisms. Thus, the environment's pollution is one of the most severe problems humanity and other forms of life face today on our planet, and this population makes severe disruption. The xenobiotic substances responsible for this pollution are numerous and diverse due to multiple human activities that can be the source. However, attention mainly focuses on fertilizers, pesticides, heavy metals, and certain petrochemicals of significant consumption, such as solvents. The purpose of this study is to highlight the reprotoxic and hepatotoxic effects of Ethylene Glycol Monoethyl Ether (EGME) on male Wistar rats. Male Wistar rats were exposed to Ethylene Glycol Monomethyl Ether alone (EGME) (500 mg/kg), combined with the aqueous extract of the pollen grains of Sinapis arvensis (P-EGME) (300 mg/kg), in addition to the control (T) and positive control (P) groups for a period of 4 weeks by gavage, to estimate the protective role of this plant against the intoxication of EGME. The results show that EGME can induce reprotoxic effects revealed by a reduction in testes and epididymis mass accompanied by decreased male fertility indicators (sperm concentration). The results also show that treatment with EGME caused a reduction in the red blood cell number, hemoglobin and hematocrit rate, white blood cells in the treated groups compared to the control groups. The present study revealed that treatment with Ethylene Glycol Monoethyl Ether (EGME) under the same experimental conditions could affect several biological markers, especially male fertility. Besides, the protective activity of the wild mustard (Sinapis arvensis) pollen in the face of cellular oxidative damage induced by EGME. So, it is suggested that this pollen could be used as a cell protector.

Concentration of spermatozoa (106/ml) in the groups of rats: T, P, EGME, P-EGME (n = 8). * P < 0.05; ** P < 0.01; *** P < 0.001.

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Published

2021-05-03

How to Cite

Hamdi, L., Djemli, S., Arkoub, F. Z., Boukarine, . R., Khelili, K., Abdennour, . C., & Tahraoui, A. (2021). The protective effect of wild mustard (&lt;em&gt;Sinapis arvensis&lt;/em&gt; L.) pollen seeds against the toxicity of a solvent (EGME) in Wistar rats. Journal of Animal Behaviour and Biometeorology, 9(3), 2130. Retrieved from https://malque.pub/ojs/index.php/jabb/article/view/45

Issue

Section

Research Article