• Abstract

    This study aims to examine the effect of synbiotic nutmeg flesh extract and L. plantarum on intestinal morphology, stress indicators and bacterial populations in broiler chickens reared at high densities. Broiler chickens (body weight 142 ± 4,71 g) were randomly divided into five treatments, and each treatment was repeated five times, namely, T0 (negative control with a normal density of 10 birds/m2), T1 (positive control with a high density of 18 birds/m2), T2, T3, and T4 with a high density of 18 birds/m2. The synbiotic nutmeg flesh extract and L. plantarum were added to the feed from the eighth day at 0.5, 1.0, and 1.5 ml/kg for T2, T3, and T4, respectively. The results of the study showed that administering synbiotic nutmeg flesh extract and L. plantarum to broiler chickens reared at high density significantly (p<0.05) increased growth performance and villous length in the duodenum, jejunum, and ileum. In addition, this synbiotic also lowers the pH in the ileum and cecum, increases lactic acid bacteria and reduces coliform bacteria in the ileum and cecum, reduces the H/L and malondialdehyde (MDA) ratio and increases superoxide dismutase (SOD). Providing synbiotic nutmeg flesh extract and L. plantarum is also able to improve the performance and stress of broiler chickens raised at high densities.

  • References

    1. Abdel-Raheem SM, Abd-Allah SM, Hassanein KM (2012) The effects of prebiotic, probiotic and synbiotic supplementation on intestinal microbial ecology and histomorphology of broiler chickens. Int. J. Agr. Vet. Med. Sci 6:277-289.
    2. Agusetyaningsih I, Widiastuti E, Wahyuni HI, Yudiarti T, Murwani R, Sartono TA, Sugiharto S (2022) Effect of encapsulated Cosmos caudatus leaf extract on the physiological conditions, immune competency, and antioxidative status of broilers at high stocking density. Ann. Anim. Sci 22:653-662. doi.org/10.2478/aoas-2021-0043
    3. Altaf MT, Mahmud A, Mehmood S (2019). Effects of supplemented growth promoters on performance and intestinal morphology in broilers reared under different stocking densities. Braz. J. Poul. Sci 21. doi.org/10.1590/1806-9061-2019-1073
    4. Altan ÖZGE, Pabuçcuoğlu A, Altan A, Konyalioğlu S, Bayraktar H (2003). Effect of heat stress on oxidative stress, lipid peroxidation and some stress parameters in broilers. Br. Poult. Sci 44:545-550. doi.org/10.1080/00071660310001618334
    5. Anjum MI, Khan AG, Azim A, Afzal M (2005). Effect of dietary supplementation of multi-strain probiotic on broiler growth performance. Pak. Vet. J 25:25-29.
    6. Biagi G, Vecchiato CG, Pinna C (2017). The Utilization of Prebiotics, Probiotics, Organic Acids and Antibiotics in Monogastric Animals. KnE Life Sci 55-62.
    7. Blajman JE, Frizzo LS, Zbrun MV, Astesana DM, Fusari ML, Soto LP, Rosmini MR, Signorini ML (2014). Probiotics and broiler growth performance: a meta-analysis of randomised controlled trials. Br. Poult. Sci 55:483-494. doi.org/10.1080/00071668.2014.931930
    8. Cengiz Ö, Köksal BH, Tatlı O, Sevim Ö, Ahsan U, Üner AG, Ulutaş PA, Beyaz D, Büyükyörük S, Yakan A, Önol AG (2015). Effect of dietary probiotic and high stocking density on the performance, carcass yield, gut microflora, and stress indicators of broilers. Poult. Sci 94:2395-2403. doi.org/10.3382/ps/pev194
    9. Dizaji BR, Hejazi S, Zakeri A (2012). Effects of dietary supplementations of prebiotics, probiotics, synbiotics and acidifiers on growth performance and organs weights of broiler chicken. Euro. J. Exp. Bio 2:2125-2129.
    10. Ferdous MF, Arefin MS, Rahman MM, Ripon MMR, Rashid MH, Sultana MR, Hossain MT, Ahammad MU, Rafiq K (2019). Beneficial effects of probiotic and phytobiotic as growth promoter alternative to antibiotic for safe broiler production. J. adv. Vet. Anim. Res 6:409-415.
    11. Hassanpour H, Moghaddam AZ, Khosravi M, Mayahi M (2013). Effects of synbiotic on the intestinal morphology and humoral immune response in broiler chickens. Livest. Sci 153:116-122. doi.org/10.1016/j.livsci.2013.02.004
    12. Ismail FSA, El-Gogary MR, El-Nadi MI (2014). Influence of vitamin E supplementation and stocking density on performance, thyroid status, some blood parameters, immunity and antioxidant status in broiler chickens. Asian J. Anim. Vet. Adv 9:702-714.
    13. Jadhav K, Sharma KS, Katoch S, Sharma VK, Mane BG (2015). Probiotics in broiler poultry feeds: A review. J. Anim. Nutr. Physiol 1:4-16.
    14. Kim H, Rather IA, Kim H, Kim S, Kim T, Jang J, Seo J, Lim J, Park YH (2015). A double-blind, placebo controlled-trial of a probiotic strain Lactobacillus sakei probio-65 for the prevention of canine atopic dermatitis. J Microbiol Biotechnol 25:1966-1969. doi.org//10.4014/jmb.1506.06065
    15. Kridtayopas C, Rakangtong C, Bunchasak C, Loongyai W (2019). Effect of prebiotic and synbiotic supplementation in diet on growth performance, small intestinal morphology, stress, and bacterial population under high stocking density condition of broiler chickens. Poult. Sci 98:4595-4605. doi.org/10.3382/ps/pez152
    16. Li XM, Zhang MH, Liu SM, Feng JH, Ma DD, Liu QX, Zhou Y, Wang XJ, Xing S (2019). Effects of stocking density on growth performance, growth regulatory factors, and endocrine hormones in broilers under appropriate environments. Poult. Sci 98:6611-6617. doi.org/10.3382/ps/pez505
    17. Magnuson, A.D., Liu, G., Sun, T., Tolba, S.A., Xi, L., Whelan, R., Lei, X.G., 2020. Supplemental methionine and stocking density affect antioxidant status, fatty acid profiles, and growth performance of broiler chickens. J. Anim. Sci. 98, skaa092. https://doi.org/10.1093/jas/skaa092.
    18. Mohammed AA, Jacobs JA, Murugesan GR, Cheng HW (2018). Effect of dietary synbiotic supplement on behavioral patterns and growth performance of broiler chickens reared under heat stress. Poult. Sci 97:1101-1108. doi.org/10.3382/ps/pex421
    19. Peng QY, Li JD, Li Z, Duan ZY, Wu YP (2016). Effects of dietary supplementation with oregano essential oil on growth performance, carcass traits and jejunal morphology in broiler chickens. Anim. Feed Sci. Technol 214:148-153. doi.org/10.1016/j.anifeedsci.2016.02.010
    20. Pourabedin M, Xu Z, Baurhoo B, Chevaux E, Zhao X (2014). Effects of mannan oligosaccharide and virginiamycin on the cecal microbial community and intestinal morphology of chickens raised under suboptimal conditions. Can. J. microbiol 60:255-266. doi.org/10.1139/cjm-2013-0899
    21. Sapsuha Y, Suprijatna E, Kismiati S, Sugiharto S (2021). The effect of nutmeg flesh (Myristica fragrans Houtt) extract on growth performance, internal organ and carcass of broiler chickens raised at high stocking density. Livest. Res. Rural Dev 33:1-7.
    22. Sapsuha Y, Hasan S, Nur A (2023a). Survivability of Lactobacillus plantarum in nutmeg (Myristica fragrans Houtt) flesh extract and its effect on the performance of broiler chicken. J. Adv. Vet. Anim. Res 10:42. doi.org/10.5455/javar.2023.j650
    23. Sapsuha Y, Hasan S, Nur A (2023b). The effect of symbiotic supplementation of nutmeg flesh extract (Myristica fragrans Houtt) and Lactobacillus plantarum on microbial and intestinal morphology of broiler chickens. Livest. Res. Rural Dev 35:1.
    24. Sapsuha Y, Suprijatna E, Kismiati S, Sugiharto S (2022). Possibility of using nutmeg flesh (Myristica fragrans houtt) extract in broiler diet to improve intestinal morphology, bacterial population, blood profile and antioxidant status of broilers under high-density condition. Agro. Res 20:1. doi.org/10.15159/ar.22.007
    25. Sarangi NR, Babu LK, Kumar A, Pradhan CR, Pati PK, Mishra JP (2016). Effect of dietary supplementation of prebiotic, probiotic, and synbiotic on growth performance and carcass characteristics of broiler chickens. Vet. World 9:313-319. doi.org/10.14202/vetworld.2016.313-319
    26. Selvam R, Saravanakumar M, Suresh S, Sureshbabu G, Sasikumar M, Prashanth D (2017). Effect of vitamin E supplementation and high stocking density on the performance and stress parameters of broilers. Braz. J. Poult. Sci 19:587-594. doi.org/10.1590/1806-9061-2016-0417
    27. Shakeri M, Shakeri M, Omidi A (2014). Effect of garlic supplementation to diet on performance and intestinal morphology of broiler chickens under high stocking density. Fac. Vet. Med. Istanbul Univ 41:212-217. doi.org/10.16988/iuvfd.2015.41064
    28. Simitzis PE, Kalogeraki E, Goliomytis M, Charismiadou MA, Triantaphyllopoulos K, Ayoutanti A, Niforou K, Hager-Theodorides AL, Deligeorgis SG (2012). Impact of stocking density on broiler growth performance, meat characteristics, behavioural components and indicators of physiological and oxidative stress. Br. Poult. Sci 53:721-730. doi.org/10.1080/00071668.2012.745930
    29. Song J, Xiao K, Ke YL, Jiao LF, Hu CH, Diao QY, Shi B, Zou XT (2014). Effect of a probiotic mixture on intestinal microflora, morphology, and barrier integrity of broilers subjected to heat stress. Poult. Sci 93:581-588. doi.org/10.3382/ps.2013-03455
    30. Sugiharto S (2016). Role of nutraceuticals in gut health and growth performance of poultry. J. Saudi Soc. Agric. Sci 15:99-111. doi.org/10.1016/j.jssas.2014.06.001
    31. Sugiharto S, Yudiarti T, Isroli I, Widiastuti E, Putra FD (2017). Effect of dietary supplementation with Rhizopus oryzae or Chrysonilia crassa on growth performance, blood profile, intestinal microbial population, and carcass traits in broilers exposed to heat stress. Arc. Anim. Breed 60:347-356. doi.org/10.5194/aab-60-347-2017
    32. Sugiharto S, Yudiarti T (2022). The effect of using acidified turmeric on some productive parameters and intestinal bacterial counts in broilers at high stocking density pens. J. Adv. Vet. Anim. Res 9:87-94. doi.org/10.5455/javar.2022.i572
    33. Sunu P, Sunarti D, Mahfudz LD, Yunianto VD (2021). Effect of synbiotic from Allium sativum and Lactobacillus acidophilus on hematological indices, antioxidative status and intestinal ecology of broiler chicken. J. Saudi Soc. Agric. Sci 20:103-110. doi.org/10.1016/j.jssas.2020.12.005
    34. Tang KL, Caffrey NP, Nóbrega DB, Cork SC, Ronksley PE, Barkema HW, Polachek AJ, Ganshhorn H, Sharma N, Kellner JD, Ghali WA (2017). Restricting the use of antibiotics in food-producing animals and its associations with antibiotic resistance in food-producing animals and human beings: a systematic review and meta-analysis. Lancet Planet. Health 1:e316-e327. doi.org/10.1016/S2542-5196(17)30141-9
    35. Thaxton JP, Dozier III, WA, Branton SL, Morgan GW, Miles DW, Roush WB, Lott BD, Vizzier-Thaxton Y (2006). Stocking density and physiological adaptive responses of broilers. Poult. Sci 85:819-824. doi.org/10.1093/ps/85.5.819
    36. Tsiouris V, Georgopoulou I, Batzios C, Pappaioannou N, Ducatelle R, Fortomaris P (2015). High stocking density as a predisposing factor for necrotic enteritis in broiler chicks. Avian Pathol 44:59-66. doi.org/10.1080/03079457.2014.1000820
    37. Wu S (2018). Effect of dietary Astragalus membranaceus polysaccharide on the growth performance and immunity of juvenile broilers. Poult. Sci 97:3489-3493. doi.org/10.3382/ps/pey220
    38. Yin LY, Wang ZY, Yang HM, Xu L, Zhang J, Xing H (2017). Effects of stocking density on growth performance, feather growth, intestinal development, and serum parameters of geese. Poult. Sci 96:3163-3168. doi.org/10.1016/j.psj.2021.101417
    39. Yuanita I, Sunarti D, Wahyuni HI, Suthama N (2019). Feeding Dayak onion (Eleutherine palmifolia) extract and Lactobacillus acidophilus mixture on blood biochemicals, meat quality characteristics and growth performance in broiler chickens. Livest. Res. Rural Dev 31:23-32.
    40. Zhang C, Zhao XH, Yang L, Chen XY, Jiang RS, Jin SH, Geng ZY (2017). Resveratrol alleviates heat stress-induced impairment of intestinal morphology, microflora, and barrier integrity in broilers. Poult. Sci 96:4325-4332. doi.org/10.3382/ps/pex266
    41. Zhao CY, Tan SX, Xiao XY, Qiu XS, Pan JQ, Tang ZX (2014). Effects of dietary zinc oxide nanoparticles on growth performance and antioxidative status in broilers. Biol. Trace Elem. Res 160:361-367. doi.org/10.1007/s12011-014-0052-2

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Copyright (c) 2023 Malque Publishing

How to cite

Sapsuha, Y., Hasan, S., & Nur, A. (2023). Effect of synbiotic from nutmeg flesh extract and <em>Lactobacillus plantarum</em> on small intestinal morphology, stress, and bacterial population of broiler chickens under high stocking density conditions. Journal of Animal Behaviour and Biometeorology, 11(4), 2023036. https://doi.org/10.31893/jabb.23036
  • Article viewed - 57
  • PDF downloaded - 11