Histomorphological and immunohistochemical studies of the ovary during dry and rainy seasons in Small East African goats (<em>Capra hircus</em>)

Jackson Ngou; Wahabu Kimaro; Claudius Luziga

doi:10.31893/avr.2023013

Keywords:

Abstract

This study aimed to investigate the histomorphological and immunohistochemical changes in the ovaries of Small East African goats during the dry and rainy seasons. A total of 180 ovaries were subjected to analysis using hematoxylin and eosin staining and immunohistochemistry for desmin and smooth muscle actin as cytoskeletal components. The results demonstrated the presence of healthy and atretic primordial, primary, secondary, and antral follicles in the ovaries during the dry and rainy seasons. In the dry season, the proportions of healthy primordial, primary, secondary, and antral follicles were 236 (95%), 85 (97%), 25 (78%), and 6 (27%), respectively, whereas the atretic follicles accounted for 12 (5%), 3 (3%), 7 (22%), and 16 (73%). Conversely, during the rainy season, 157 (99%), 61 (98%), 48 (93%), and 31 (92%) were classified as healthy follicles, while 2 (1%), 1 (2%), 4 (7%), and 3 (8%) were categorized as atretic. There was a significant difference in healthy and atretic follicles between the dry and rainy seasons (P < 0.05). Immunohistochemical analysis revealed positive staining for both desmin and smooth muscle actin in the theca cells of secondary and antral follicles, cortical stroma, and tunica media of blood vessels in healthy and atretic follicles during dry and rainy seasons. However, staining was not observed in the granulosa cells and oocytes. Overall, this study indicates that most antral follicles undergo obliterated follicular atresia during the dry season.
References
1. Ariyaratna HBS, Gunawardana VK (1997) Morphology and morphometry of ovarian follicles in the goat. Small Ruminant Research 26:123–129.
2. Bari M, Kabir M, Sarker M, Khan A, Momiruzzaman M (2012) Morphometric analysis of ovarian follicles of Black Bengal goats during winter and summer season. Bangladesh Journal of Animal Science 40:51–55. doi:10.3329/bjas.v40i1-2.10791.
3. Bijna M, Karthiayini K, Lucy, KM (2018) Morphometry and Histology of Ovaries in Malabari Goats. International Journal of Science and Research (IJSR) 7:291–293. doi: 10.21275/ART20179048.
4. Das GK, Khan FA (2010) Summer anoestrus in buffalo - A review. Reproduction in Domestic Animals. doi: 10.1111/j.1439-0531.2010.01598.x.
5. Emara S, Hassanin A, Elnagar H (2019) Histological and histochemical changes of the ovary of goat during autumn and summer seasons. Journal of Current Veterinary Research 1:47–54. doi: 10.21608/jcvr.2019.57027.
6. Helfand BT, Chang L, Goldman RD (2004) Intermediate filaments are dynamic and motile elements of cellular architecture. Journal of Cell Science 117:133–141. doi: 10.1242/jcs.00936.
7. Islam MR, Khandoker MA, Afroz S, Rahman MG, Khan RI (2007) Qualitative and quantitative analysis of goat ovaries, follicles and oocytes in view of in vitro production of embryos. Journal of Zhejiang University Science 8:465–469.
8. Khan-dawood FS, Dawood MY, Tabibzadeh S (1996) Immunohistochemical Analysis of the Microanatomy of Primate Ovary. Biology of Rproduction 54:734–742.
9. Madekurozwa MC, Oke BO, Akinloye AK (2010) The immunohistochemical localization of desmin and smooth muscle actin in the ovary of the African giant rat (Cricetomys gambianus) during the oestrous cycle. Journal of Veterinary Medicine Series C: Anatomia Histologia Embryologia 39:81–86. doi: 10.1111/j.1439-0264.2009.00983.x.
10. Madekurozwa MC, Kimaro WH (2006) A Morphological and Immunohistochemical Study of Healthy and Atretic Follicles in the Ovary of the Sexually Immature Ostrich (Struthio camelus) Anatomia Histologia Embryologia 35:253–258. doi: 10.1111/j.1439-0264.2005.00675.x.
11. Marc C, Nassauw LV (2005) Immunohistochemical demonstration of actin and desmin by monoclonal antibodies in the ovary of the rat Research Journal of Medical Sciences 15:555-558.
12. Marettová E, Maretta M (2002) Demonstration of intermediate filaments in sheep ovary. Acta Histochemica 104:431–434. doi: 10.1078/0065-1281-00672.
13. Paulin D, Li Z (2004) Desmin : a major intermediate filament protein essential for the structural integrity and function of muscle. Experimental Cell Research 301:1–7. doi: 10.1016/j.yexcr.2004.08.004.
14. Salvetti NR, Gimeno J, Lorente JA (2004) Expression of Cytoskeletal Proteins in the Follicular Wall of Induced Ovarian Cysts. Cells Tissue Organs 178:117–125. doi: 10.1159/000081721.
15. Slaoui M, Fiette L (2011) Histopathology procedures: from tissue sampling to histopathological evaluation. Methods in Molecular Biology 691:69–82. doi: 10.1007/978-1-60761-849-2_4.
16. Tanzania Meteorological Authority (2021) Statement on the Status of Climate change in Tanzania. https://www.meteo.go.tz/.
17. Teh APP, Izzati UZ, Fuke KMN, Kitahara THG (2018) Histological and immunohistochemical evaluation of granulosa cells during different stages of folliculogenesis in bovine ovaries. Reproduction in Domestic Animals 4:1–13. doi: 10.1111/rda.13132.
18. Van Nassauw LM, Callebaut F, Harrison G, Daneels M (1989) Immunohistochemical localization of desmin in the quail ovary, demonstration of a suspensory apparatus. Histochemistry 90:371–377.
19. Van Nassauw L, Callebaut M (1991) Structural and immunohistochemical aspects of the postovulatory follicle in Japanese quail. The Anatomical Record 229:27–30. doi: 10.1002/ar.1092290105.
20. Wendl J, Ebach K, Rodler D, Kenngott RAM (2012) Immunocytochemical Localization of Cytoplasmic and Nuclear Intermediate Filaments in the Bovine Ovary during Folliculogenesis. Journal of Veterinary Medicine Series C: Anatomia Histologia Embryologia 41:190–201. doi: 10.1111/j.1439-0264.2011.01123.x.

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How to cite

Ngou, J., Kimaro, W., & Luziga, C. (2023). Histomorphological and immunohistochemical studies of the ovary during dry and rainy seasons in Small East African goats (<em>Capra hircus</em>). Applied Veterinary Research, 2(3), 2023013. https://doi.org/10.31893/avr.2023013

[1] Ariyaratna HBS, Gunawardana VK (1997) Morphology and morphometry of ovarian follicles in the goat. Small Ruminant Research 26:123–129.

[2] Bari M, Kabir M, Sarker M, Khan A, Momiruzzaman M (2012) Morphometric analysis of ovarian follicles of Black Bengal goats during winter and summer season. Bangladesh Journal of Animal Science 40:51–55. doi:10.3329/bjas.v40i1-2.10791.

[3] Bijna M, Karthiayini K, Lucy, KM (2018) Morphometry and Histology of Ovaries in Malabari Goats. International Journal of Science and Research (IJSR) 7:291–293. doi: 10.21275/ART20179048.

[4] Das GK, Khan FA (2010) Summer anoestrus in buffalo - A review. Reproduction in Domestic Animals. doi: 10.1111/j.1439-0531.2010.01598.x.

[5] Emara S, Hassanin A, Elnagar H (2019) Histological and histochemical changes of the ovary of goat during autumn and summer seasons. Journal of Current Veterinary Research 1:47–54. doi: 10.21608/jcvr.2019.57027.

[6] Helfand BT, Chang L, Goldman RD (2004) Intermediate filaments are dynamic and motile elements of cellular architecture. Journal of Cell Science 117:133–141. doi: 10.1242/jcs.00936.

[7] Islam MR, Khandoker MA, Afroz S, Rahman MG, Khan RI (2007) Qualitative and quantitative analysis of goat ovaries, follicles and oocytes in view of in vitro production of embryos. Journal of Zhejiang University Science 8:465–469.

[8] Khan-dawood FS, Dawood MY, Tabibzadeh S (1996) Immunohistochemical Analysis of the Microanatomy of Primate Ovary. Biology of Rproduction 54:734–742.

[9] Madekurozwa MC, Oke BO, Akinloye AK (2010) The immunohistochemical localization of desmin and smooth muscle actin in the ovary of the African giant rat (Cricetomys gambianus) during the oestrous cycle. Journal of Veterinary Medicine Series C: Anatomia Histologia Embryologia 39:81–86. doi: 10.1111/j.1439-0264.2009.00983.x.

[10] Madekurozwa MC, Kimaro WH (2006) A Morphological and Immunohistochemical Study of Healthy and Atretic Follicles in the Ovary of the Sexually Immature Ostrich (Struthio camelus) Anatomia Histologia Embryologia 35:253–258. doi: 10.1111/j.1439-0264.2005.00675.x.

[11] Marc C, Nassauw LV (2005) Immunohistochemical demonstration of actin and desmin by monoclonal antibodies in the ovary of the rat Research Journal of Medical Sciences 15:555-558.

[12] Marettová E, Maretta M (2002) Demonstration of intermediate filaments in sheep ovary. Acta Histochemica 104:431–434. doi: 10.1078/0065-1281-00672.

[13] Paulin D, Li Z (2004) Desmin : a major intermediate filament protein essential for the structural integrity and function of muscle. Experimental Cell Research 301:1–7. doi: 10.1016/j.yexcr.2004.08.004.

[14] Salvetti NR, Gimeno J, Lorente JA (2004) Expression of Cytoskeletal Proteins in the Follicular Wall of Induced Ovarian Cysts. Cells Tissue Organs 178:117–125. doi: 10.1159/000081721.

[15] Slaoui M, Fiette L (2011) Histopathology procedures: from tissue sampling to histopathological evaluation. Methods in Molecular Biology 691:69–82. doi: 10.1007/978-1-60761-849-2_4.

[16] Tanzania Meteorological Authority (2021) Statement on the Status of Climate change in Tanzania. https://www.meteo.go.tz/.

[17] Teh APP, Izzati UZ, Fuke KMN, Kitahara THG (2018) Histological and immunohistochemical evaluation of granulosa cells during different stages of folliculogenesis in bovine ovaries. Reproduction in Domestic Animals 4:1–13. doi: 10.1111/rda.13132.

[18] Van Nassauw LM, Callebaut F, Harrison G, Daneels M (1989) Immunohistochemical localization of desmin in the quail ovary, demonstration of a suspensory apparatus. Histochemistry 90:371–377.

[19] Van Nassauw L, Callebaut M (1991) Structural and immunohistochemical aspects of the postovulatory follicle in Japanese quail. The Anatomical Record 229:27–30. doi: 10.1002/ar.1092290105.

[20] Wendl J, Ebach K, Rodler D, Kenngott RAM (2012) Immunocytochemical Localization of Cytoplasmic and Nuclear Intermediate Filaments in the Bovine Ovary during Folliculogenesis. Journal of Veterinary Medicine Series C: Anatomia Histologia Embryologia 41:190–201. doi: 10.1111/j.1439-0264.2011.01123.x.

Applied Veterinary Research

Research Article | Vol. 2 Issue 3 (2023), e2023013 | Published in 13 August 2023

Histomorphological and immunohistochemical studies of the ovary during dry and rainy seasons in Small East African goats (Capra hircus)

Abstract

References

How to cite