Genetic polymorphism of the BMP15 gene in West African sheep (Fulani) breed in Sudan

Khalied  H. Elshareef; Hassan H. Musa; Saber Y. Adam; Jaafar  S. Fedailb; Demin  Cai; Abdelkareem  A. Ahmed

doi:10.31893/avr.2023009

Keywords:

BMP 15 gene

genetic polymorphism

West African sheep

Abstract

Genetic polymorphism studies in domestic animals aim at assessing genetic variations within and across breeds mainly for preservation purposes. The bone morphogenetic protein 15 (BMP 15) gene is a growth factor and a member of the transforming growth factor (TGFβ) superfamily specifically expressed in oocytes. One of the very exciting and perhaps crucial actions of BMP-15 is its ability to inhibit Follicle Stimulating Hormone (FSH) receptor expression. The West African sheep originated in the West African republics. Three strains were named after a person (Omer Uda) conveyed to Sudan in 1930. These breeds include (Uda, Sankara, and Weila) strains. They are habitually raised to gather as mixed pastoral flocks and trailed altered paths during the summer and autumn. Genetic polymorphism of the BMP 15 gene in Sudanese sheep is not reported. Consequently, this work aimed to study the genetic polymorphism in BMP-15 in West African sheep breeds. Blood samples were taken from thirty ewes of West African sheep. DNA samples were isolated from blood samples using the Guanidine chloroform method, and the quality of the DNA was checked on 2% agarose gel electrophoresis. Mutation in the BMP15 gene was screened using the forced PCR-RFLP technique. The fundamental discovery of the present study was the nonexistence of polymorphism at the BMP15 gene in West African sheep. The nucleotide sequence of the PCR product (141 bp) revealed that the C nucleotide has not changed to T at this locus (C-T) of the BMP15 gene in all samples.
References
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3. Chawala AR, Sanchez-Molano E, Dewhurst RJ, Peters A, Chagunda MGG, Banos G (2021) Breeding strategies for improving smallholder dairy cattle productivity in Sub-Saharan Africa. Journal of Animal Breeding and Genetics 138:668-687.
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5. Ciulla T, SR, Hauser S (1988) A simple method for DNA purification from peripheral blood. Analytical Biochemistry 174:485-488.
6. Davis GH (2005) Major genes affecting ovulation rate in sheep. Genetics, selection, evolution: GSE 37 Suppl 1:S11-23.
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9. El Fiky ZA, Hassan GM, Nassar MI (2017) Genetic polymorphism of growth differentiation factor 9 (GDF9) gene related to fecundity in two Egyptian sheep breeds. J Assist Reprod Genet 34:1683-1690.
10. Elkorshy N, Mahrous K, Salem LM (2013) Genetic polymorphism detection in four genes in Egyptian and Saudi sheep breeds. World Applied Sciences Journal 27:33-43.
11. Emori, C., Sugiura, K., 2014. Role of oocyte-derived paracrine factors in follicular development. Animal Science Journal 85:627-633.
12. Esen gursel F, Akis I, Durak H, Mengi A, Öztabak (2011) Determination of BMP-15, BMPR-1B and GDF-9 gene mutations of the indigenous sheep breeds in Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 17:725-729.
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15. Hanrahan JP, SM Gregan, P Mulsant, M MullenGH Davis, R Powell and SM Galloway (2004b) Mutations in the genes for oocyte-derived growthfactors GDF9 and BMP15 are associated with bothincreased ovulation rate and sterility inCambridge and Belclare sheep (Ovis aries). Biol Reprod 70:900-909.
16. Hua, G-H, et al. (2008) None of polymorphism of ovine fecundity major genes FecB and FecX was tested in goat. Animal Reproduction Science 108:279-286.
17. Jamshidi R, MM, Hafezeya H (2009) Application of PCR-RFLP technique to determineBMP-15 gene polymorphism in Sangsari sheep breed of Iran. J Anim Vet Adv 8:1906–10.
18. Juengel JL, Davis GH, McNatty KP (2013) Using sheep lines with mutations in single genes to better understand ovarian function. REPRODUCTION 146:R111-R123.
19. Juengel JL, O'Connell AR, French MC, Proctor LE, Wheeler R, Farquhar PA, Dodds KG, Galloway SM, Johnstone PD, Davis GH (2011) Identification of a Line of Sheep Carrying a Putative Autosomal Gene Increasing Ovulation Rate in Sheep That Does Not Appear to Interact with Mutations in the Transforming Growth Factor Beta Superfamily. Biology of Reproduction 85:113-120.
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23. Lin J, DZ, Qin C, Wang J, Ran X (2007) Polymorphism of BMP15 gene in Guizhou white goats. Anim Hus Vet Med 39:21-24.
24. Lôbo AMBO, Lôbo RNB, Paiva SR, de Oliveira SMP, Facó O (2009) Genetic parameters for growth, reproductive and maternal traits in a multibreed meat sheep population. Genet Mol Biol 32:761-770.
25. Ma S, Ji X, Cang M, Wang J, Yu H, Liu Y, Zhang W, Wu Y, Zhao S, Cao G, Tong B (2022) Association analysis between novel variants in LEPR gene and litter size in Mongolia and ujimqin sheep breeds. Theriogenology 183:79-89.
26. Magro-Lopez E, Muñoz-Fernández MÁ (2021) The Role of BMP Signaling in Female Reproductive System Development and Function. Int J Mol Sci 22: 11927.
27. McMahon HE, Hashimoto O, Mellon PL, Shimasaki S (2008a) Oocyte-specific overexpression of mouse bone morphogenetic protein-15 leads to accelerated folliculogenesis and an early onset of acyclicity in transgenic mice. Endocrinology 149:2807-2815.
28. McMahon HE, Sharma S, Shimasaki S (2008b) Phosphorylation of bone morphogenetic protein-15 and growth and differentiation factor-9 plays a critical role in determining agonistic or antagonistic functions. Endocrinology 149:812-817.
29. Mohammad Mehdi Kasiriyan, SHHaNH (2011) Genetic polymorphism BMP15 and GDF9 genes in Sangsari sheep of Iran. International Journal of Genetics and Molecular Biology 3:31-34.
30. Moradband F, Rahimi-Mianji G, Gholizadeh M (2011) Association of Polymorphisms in Fecundity Genes of GDF9, BMP15 and BMP15-1B with Litter Size in Iranian Baluchi Sheep. Asian-Australasian journal of animal sciences 24.
31. Musa H, AH, S, amin FM, GH, C, Mekki D, BC, L (2005a) Evaluation of West African Sheep under Two Production Systems. Journal of Animal and Veterinary Advances.
32. Musa H, AH, S, El amin FM, GH, C, Mekki D, BC, L (2005b) Evaluation of West African Sheep under Two Production Systems.
33. Niu Z-G, Qin J, Jiang Y, Ding X-D, Ding Y-G, Tang S, Shi H-C (2021) The Identification of Mutation in BMP15 Gene Associated with Litter Size in Xinjiang Cele Black Sheep. Animals 11:668.
34. Osman NM, Shafey HI, Abdelhafez MA, Sallam AM, Mahrous KF (2021) Genetic variations in the Myostatin gene affecting growth traits in sheep. Veterinary world 14:475-482.
35. Otsuka F, McTavish KJ, Shimasaki S (2011) Integral role of GDF-9 and BMP-15 in ovarian function. Mol Reprod Dev 78:9-21.
36. Patiño LC, Walton KL, Mueller TD, Johnson KE, Stocker W, Richani D, Agapiou D, Gilchrist RB, Laissue P, Harrison CA (2017) BMP15 Mutations Associated With Primary Ovarian Insufficiency Reduce Expression, Activity, or Synergy With GDF9. The Journal of Clinical Endocrinology & Metabolism 102:1009-1019.
37. Persani L, Rossetti R, Di Pasquale E, Cacciatore C, Fabre S (2014) The fundamental role of bone morphogenetic protein 15 in ovarian function and its involvement in female fertility disorders. Human reproduction update 20:869-883.
38. Polley S, De S, Brahma B, Mukherjee A, PV, V, Batabyal S, Arora JS, Pan S, Kumar Samanta A, Kumar Datta T, Goswami S (2010) Polymorphism of BMPR1B, BMP15 and GDF9 fecundity genes in prolific Garole sheep.
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43. Rossetti R, Di Pasquale E, Marozzi A, Bione S, Toniolo D, Grammatico P, Nelson LM, Beck-Peccoz P, Persani L (2009) BMP15 mutations associated with primary ovarian insufficiency cause a defective production of bioactive protein. Hum Mutat 30:804-810.
44. Sato E (2015) Intraovarian control of selective follicular growth and induction of oocyte maturation in mammals. Proc Jpn Acad Ser B Phys Biol Sci 91:76-91.
45. Tong B, Wang J, Cheng Z, Liu J, Wu Y. Li Y, Bai C, Zhao S, Yu H, Li G (2020) Novel Variants in GDF9 Gene Affect Promoter Activity and Litter Size in Mongolia Sheep. Genes 11.
46. Wang W, Liu S, Li F, Pan X, Li C, Zhang X, Ma Y, La Y, Xi R, Li T (2015) Polymorphisms of the Ovine BMPR-IB, BMP-15 and FSHR and Their Associations with Litter Size in Two Chinese Indigenous Sheep Breeds. Int J Mol Sci 16:11385-11397.
47. Yāmi Ā, Merkel RC, Sheep E, Program GPI (2008) Sheep and Goat Production Handbook for Ethiopia. Ethiopia Sheep and Goat Productivity Improvement Program.
48. Yuan Z, Zhang J, Li W, Wang W, Li F, Yue X (2019) Association of Polymorphisms in Candidate Genes with the Litter Size in Two Sheep Breeds. Animals 9:958.
49. Zamani P, NSلR, Ahmadi A, Dashti F, Abdoli R (2015) A new mutation in exon 2 of the bone morphogenetic protein 15 gene is associated with increase inprolificacy of Mehrabanand Lori sheep. Trop. Animal Health Prod. 47:855–60
50. Zare Y, N-JA, Rahimi G (2007) Detection ofpolymorphisms in two point of gene associated with Twinning (BMP15) in Shal sheep. The 5th National Biotechnology Congress of Iran. pp. 483.
51. Zuo B, Qian H, Wang Z, Wang X, Nisa N, Bayier A, Ying S, Hu X, Gong C, Guo Z, Wang F (2013) A Study on BMPR-IB Genes of Bayanbulak Sheep. Asian-Australasian journal of animal sciences 26:36-42.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to cite

H. Elshareef, K. ., Musa, H. H., Y. Adam, S., S. Fedailb, J. ., Cai, D. ., & A. Ahmed, A. . (2023). Genetic polymorphism of the BMP15 gene in West African sheep (Fulani) breed in Sudan. Applied Veterinary Research, 2(2), 2023009. https://doi.org/10.31893/avr.2023009

[1] Abdoli R, Zamani P, Mirhoseini SZ, Ghavi Hossein-Zadeh N, Nadri S (2016) A review on prolificacy genes in sheep. Reproduction in domestic animals = Zuchthygiene 51:631-637.

[2] Afkhami F, Shahbazi S, Farzadi L, Danaei S (2022) Novel bone morphogenetic protein 15 (BMP15) gene variants implicated in premature ovarian insufficiency. Reprod Biol Endocrinol 20:42-42.

[3] Chawala AR, Sanchez-Molano E, Dewhurst RJ, Peters A, Chagunda MGG, Banos G (2021) Breeding strategies for improving smallholder dairy cattle productivity in Sub-Saharan Africa. Journal of Animal Breeding and Genetics 138:668-687.

[4] Chu MX LZ, Jiao CL, He YQ, Fang L, Ye SCJ (2007) Mutations in BMPR-IB and BMP-15 genes are associated with litter size in small tailed Han sheep (Ovisaries). Animal Science 85:598-603.

[5] Ciulla T, SR, Hauser S (1988) A simple method for DNA purification from peripheral blood. Analytical Biochemistry 174:485-488.

[6] Davis GH (2005) Major genes affecting ovulation rate in sheep. Genetics, selection, evolution: GSE 37 Suppl 1:S11-23.

[7] Demars J, Fabre S, Sarry J, Rossetti R, Gilbert H, Persani L, Tosser-Klopp G, Mulsant P, Nowak Z, Drobik W, Martyniuk E, Bodin L (2013) Genome-wide association studies identify two novel BMP15 mutations responsible for an atypical hyperprolificacy phenotype in sheep. PLoS Genet 9:e1003482-e1003482.

[8] Di R, Wang F, Yu P, Wang X, He X, Mwacharo JM, Pan L, Chu M (2021) Detection of Novel Variations Related to Litter Size in BMP15 Gene of Luzhong Mutton Sheep (Ovis aries). Animals (Basel) 11.

[9] El Fiky ZA, Hassan GM, Nassar MI (2017) Genetic polymorphism of growth differentiation factor 9 (GDF9) gene related to fecundity in two Egyptian sheep breeds. J Assist Reprod Genet 34:1683-1690.

[10] Elkorshy N, Mahrous K, Salem LM (2013) Genetic polymorphism detection in four genes in Egyptian and Saudi sheep breeds. World Applied Sciences Journal 27:33-43.

[11] Emori, C., Sugiura, K., 2014. Role of oocyte-derived paracrine factors in follicular development. Animal Science Journal 85:627-633.

[12] Esen gursel F, Akis I, Durak H, Mengi A, Öztabak (2011) Determination of BMP-15, BMPR-1B and GDF-9 gene mutations of the indigenous sheep breeds in Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 17:725-729.

[13] Esmaeili-Fard SM, Gholizadeh M, Hafezian SH, Abdollahi-Arpanahi R (2021) Genes and Pathways Affecting Sheep Productivity Traits: Genetic Parameters, Genome-Wide Association Mapping, and Pathway Enrichment Analysis. Front Genet 12.

[14] Hanrahan JP, SM Gregan, P Mulsant, M MullenGH Davis, R Powell and S.M. and Galloway (2004a) Mutations in the genes for oocyte-derived growthfactors GDF9 and BMP15 are associated with bothincreased ovulation rate and sterility inCambridge and Belclare sheep (Ovis aries). Biol.Reprod 70:900-909.

[15] Hanrahan JP, SM Gregan, P Mulsant, M MullenGH Davis, R Powell and SM Galloway (2004b) Mutations in the genes for oocyte-derived growthfactors GDF9 and BMP15 are associated with bothincreased ovulation rate and sterility inCambridge and Belclare sheep (Ovis aries). Biol Reprod 70:900-909.

[16] Hua, G-H, et al. (2008) None of polymorphism of ovine fecundity major genes FecB and FecX was tested in goat. Animal Reproduction Science 108:279-286.

[17] Jamshidi R, MM, Hafezeya H (2009) Application of PCR-RFLP technique to determineBMP-15 gene polymorphism in Sangsari sheep breed of Iran. J Anim Vet Adv 8:1906–10.

[18] Juengel JL, Davis GH, McNatty KP (2013) Using sheep lines with mutations in single genes to better understand ovarian function. REPRODUCTION 146:R111-R123.

[19] Juengel JL, O'Connell AR, French MC, Proctor LE, Wheeler R, Farquhar PA, Dodds KG, Galloway SM, Johnstone PD, Davis GH (2011) Identification of a Line of Sheep Carrying a Putative Autosomal Gene Increasing Ovulation Rate in Sheep That Does Not Appear to Interact with Mutations in the Transforming Growth Factor Beta Superfamily. Biology of Reproduction 85:113-120.

[20] Kalds P, Zhou S, Cai B, Liu J, Wang Y, Petersen B, Sonstegard T, Wang X, Chen Y (2019) Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era. Front Genet 10.

[21] Kumar P, Sharma S, Kumar R, Rajan A (2013) Genetics of ovulation rate in farm animals. Veterinary World 6:833-838.

[22] Lassoued N, Benkhlil Z, Woloszyn F, Rejeb A, Aouina M, Rekik M, Fabre S, Bedhiaf-Romdhani S (2017) FecXBara Novel BMP15 mutation responsible for prolificacy and female sterility in Tunisian Barbarine Sheep. BMC Genetics 18:43.

[23] Lin J, DZ, Qin C, Wang J, Ran X (2007) Polymorphism of BMP15 gene in Guizhou white goats. Anim Hus Vet Med 39:21-24.

[24] Lôbo AMBO, Lôbo RNB, Paiva SR, de Oliveira SMP, Facó O (2009) Genetic parameters for growth, reproductive and maternal traits in a multibreed meat sheep population. Genet Mol Biol 32:761-770.

[25] Ma S, Ji X, Cang M, Wang J, Yu H, Liu Y, Zhang W, Wu Y, Zhao S, Cao G, Tong B (2022) Association analysis between novel variants in LEPR gene and litter size in Mongolia and ujimqin sheep breeds. Theriogenology 183:79-89.

[26] Magro-Lopez E, Muñoz-Fernández MÁ (2021) The Role of BMP Signaling in Female Reproductive System Development and Function. Int J Mol Sci 22: 11927.

[27] McMahon HE, Hashimoto O, Mellon PL, Shimasaki S (2008a) Oocyte-specific overexpression of mouse bone morphogenetic protein-15 leads to accelerated folliculogenesis and an early onset of acyclicity in transgenic mice. Endocrinology 149:2807-2815.

[28] McMahon HE, Sharma S, Shimasaki S (2008b) Phosphorylation of bone morphogenetic protein-15 and growth and differentiation factor-9 plays a critical role in determining agonistic or antagonistic functions. Endocrinology 149:812-817.

[29] Mohammad Mehdi Kasiriyan, SHHaNH (2011) Genetic polymorphism BMP15 and GDF9 genes in Sangsari sheep of Iran. International Journal of Genetics and Molecular Biology 3:31-34.

[30] Moradband F, Rahimi-Mianji G, Gholizadeh M (2011) Association of Polymorphisms in Fecundity Genes of GDF9, BMP15 and BMP15-1B with Litter Size in Iranian Baluchi Sheep. Asian-Australasian journal of animal sciences 24.

[31] Musa H, AH, S, amin FM, GH, C, Mekki D, BC, L (2005a) Evaluation of West African Sheep under Two Production Systems. Journal of Animal and Veterinary Advances.

[32] Musa H, AH, S, El amin FM, GH, C, Mekki D, BC, L (2005b) Evaluation of West African Sheep under Two Production Systems.

[33] Niu Z-G, Qin J, Jiang Y, Ding X-D, Ding Y-G, Tang S, Shi H-C (2021) The Identification of Mutation in BMP15 Gene Associated with Litter Size in Xinjiang Cele Black Sheep. Animals 11:668.

[34] Osman NM, Shafey HI, Abdelhafez MA, Sallam AM, Mahrous KF (2021) Genetic variations in the Myostatin gene affecting growth traits in sheep. Veterinary world 14:475-482.

[35] Otsuka F, McTavish KJ, Shimasaki S (2011) Integral role of GDF-9 and BMP-15 in ovarian function. Mol Reprod Dev 78:9-21.

[36] Patiño LC, Walton KL, Mueller TD, Johnson KE, Stocker W, Richani D, Agapiou D, Gilchrist RB, Laissue P, Harrison CA (2017) BMP15 Mutations Associated With Primary Ovarian Insufficiency Reduce Expression, Activity, or Synergy With GDF9. The Journal of Clinical Endocrinology & Metabolism 102:1009-1019.

[37] Persani L, Rossetti R, Di Pasquale E, Cacciatore C, Fabre S (2014) The fundamental role of bone morphogenetic protein 15 in ovarian function and its involvement in female fertility disorders. Human reproduction update 20:869-883.

[38] Polley S, De S, Brahma B, Mukherjee A, PV, V, Batabyal S, Arora JS, Pan S, Kumar Samanta A, Kumar Datta T, Goswami S (2010) Polymorphism of BMPR1B, BMP15 and GDF9 fecundity genes in prolific Garole sheep.

[39] Qin Y, Tang T, Li W, Liu Z, Yang X, Shi X, Sun G, Liu X, Wang M, Liang X, Cong P, Mo D, Liu X, Chen Y, He Z (2019) Bone Morphogenetic Protein 15 Knockdown Inhibits Porcine Ovarian Follicular Development and Ovulation. Frontiers in Cell and Developmental Biology 7.

[40] Quan K, Li J, Han H, Wei H, Zhao J, Si HA, Zhang X, Zhang D (2020) Review of Huang-huai sheep, a new multiparous mutton sheep breed first identified in China. Tropical animal health and production 53:35-35.

[41] Rexroad, C., Vallet, J., Matukumalli, L.K., Reecy, J., Bickhart, D., Blackburn, H., Boggess, M., Cheng, H., Clutter A, Cockett N, Ernst C, Fulton JE, Liu J, Lunney J, Neibergs H, Purcell C, Smith TPL, Sonstegard T, Taylor J, Telugu B, Eenennaam AV, Tassell CPV, Wells K (2019) Genome to Phenome: Improving Animal Health, Production, and Well-Being – A New USDA Blueprint for Animal Genome Research. Front Genet 10:2018–2027.

[42] Rosatia E Mousab, Van Vleckc Youngc (2002) Genetic Parameters of Reproductive Traits in Sheep. elsevier Small Ruminant Research Genetic parameters of reproductive traits in sheep 65–74.

[43] Rossetti R, Di Pasquale E, Marozzi A, Bione S, Toniolo D, Grammatico P, Nelson LM, Beck-Peccoz P, Persani L (2009) BMP15 mutations associated with primary ovarian insufficiency cause a defective production of bioactive protein. Hum Mutat 30:804-810.

[44] Sato E (2015) Intraovarian control of selective follicular growth and induction of oocyte maturation in mammals. Proc Jpn Acad Ser B Phys Biol Sci 91:76-91.

[45] Tong B, Wang J, Cheng Z, Liu J, Wu Y. Li Y, Bai C, Zhao S, Yu H, Li G (2020) Novel Variants in GDF9 Gene Affect Promoter Activity and Litter Size in Mongolia Sheep. Genes 11.

[46] Wang W, Liu S, Li F, Pan X, Li C, Zhang X, Ma Y, La Y, Xi R, Li T (2015) Polymorphisms of the Ovine BMPR-IB, BMP-15 and FSHR and Their Associations with Litter Size in Two Chinese Indigenous Sheep Breeds. Int J Mol Sci 16:11385-11397.

[47] Yāmi Ā, Merkel RC, Sheep E, Program GPI (2008) Sheep and Goat Production Handbook for Ethiopia. Ethiopia Sheep and Goat Productivity Improvement Program.

[48] Yuan Z, Zhang J, Li W, Wang W, Li F, Yue X (2019) Association of Polymorphisms in Candidate Genes with the Litter Size in Two Sheep Breeds. Animals 9:958.

[49] Zamani P, NSلR, Ahmadi A, Dashti F, Abdoli R (2015) A new mutation in exon 2 of the bone morphogenetic protein 15 gene is associated with increase inprolificacy of Mehrabanand Lori sheep. Trop. Animal Health Prod. 47:855–60

[50] Zare Y, N-JA, Rahimi G (2007) Detection ofpolymorphisms in two point of gene associated with Twinning (BMP15) in Shal sheep. The 5th National Biotechnology Congress of Iran. pp. 483.

[51] Zuo B, Qian H, Wang Z, Wang X, Nisa N, Bayier A, Ying S, Hu X, Gong C, Guo Z, Wang F (2013) A Study on BMPR-IB Genes of Bayanbulak Sheep. Asian-Australasian journal of animal sciences 26:36-42.

Abstract

References

How to cite