Histological evaluation of Rhipicephalus (Boophilus) microplus tick ovaries subjected to cobalt-60 ionizing radiation

Authors

  • Alexandre Antonio Pasqualini Institute of Energy and Nuclear Research (IPEN), Department of Radiation Technology Center, University of São Paulo. Box: 05508-000, São Paulo,SP, Brazil. https://orcid.org/0000-0002-6489-0124
  • Valter Arthur Institute of Energy and Nuclear Research (IPEN), Department of Radiation Technology Center, University of São Paulo. Box: 05508-000, São Paulo,SP, Brazil. https://orcid.org/0000-0003-3521-9136
  • Michelen Barbosa Schiavolin Biomedical scientist and research fellow at FAPESP (2017/18853-0).
  • Marina Rodrigues de Abreu Department of Biology at Instituto de Biociências, Universidade Estadual Paulista (UNESP), Rio Claro, SP, Brazil.
  • Maria Izabel Camargo-Mathias Department of Biology at Instituto de Biociências, Universidade Estadual Paulista (UNESP), Rio Claro, SP, Brazil.

DOI:

https://doi.org/10.31893/avr.2022007

Keywords:

cobalt-60, histology, ovaries, tick

Abstract

Females of Rhipicephalus (Boophilus) microplus ticks ingurgitated and in the prepost phase were submitted to doses of 0, 5, 10, 15, 20 and 25 Gy of Cobalt-60 with the aim of evaluating their effects on ovarian morphohistology with consequent establishment of the degree of damage caused to the reproductive organs of this species. For this purpose, 24 hours after exposure to Co-60 (142 Gy/h) the ectoparasites were dissected and had their ovaries removed and prepared for histology with haematoxylin and eosin staining. The results obtained showed that the 5 Gy dose radiation had a morphological aspect similar to the control standard adopted. The histological sections related to doses of 10 and 15 Gy did not cause many changes in the ovaries, except that changes were observed in the calf granules (size, distribution and staining pattern), as well as the extensive presence of cytoplasmic vacuoles in the ovocytes, especially in the region that makes contact with the ovocyte/pedicel, suggesting the occurrence of changes also in the physiology of the organ. In the ovaries exposed to doses of 20 and 25 Gy, severe alterations were observed in the organ as a whole, as well as in the germ cells (oocytes) which suffered alterations in size and shape, distribution of calf granules, involvement of the DNA present in the germinal vesicle (oocyte nuclei), besides the extensive cytoplasmic vacuolization, alterations which made the maturation of these cells impossible and consequently inhibited the production of new individuals.

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Pool of ovaries removed from females of each treatment to be processed for histology.

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Published

14-05-2022

How to Cite

Pasqualini, A. A., Arthur, V., Schiavolin, M. B., Abreu, M. R. de, & Camargo-Mathias, M. I. (2022). Histological evaluation of <em>Rhipicephalus (Boophilus) microplus</em> tick ovaries subjected to cobalt-60 ionizing radiation. Applied Veterinary Research, 1(2), e2022007. https://doi.org/10.31893/avr.2022007

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Research Article