Keywords:
cobalt-60
histology
ovaries
tick

  • Abstract

    Females of Rhipicephalus (Boophilus) microplus ticks ingurgitated and in the prepost phase were submitted to doses of 0, 5, 10, 15, 20 and 25 Gy of Cobalt-60 with the aim of evaluating their effects on ovarian morphohistology with consequent establishment of the degree of damage caused to the reproductive organs of this species. For this purpose, 24 hours after exposure to Co-60 (142 Gy/h) the ectoparasites were dissected and had their ovaries removed and prepared for histology with haematoxylin and eosin staining. The results obtained showed that the 5 Gy dose radiation had a morphological aspect similar to the control standard adopted. The histological sections related to doses of 10 and 15 Gy did not cause many changes in the ovaries, except that changes were observed in the calf granules (size, distribution and staining pattern), as well as the extensive presence of cytoplasmic vacuoles in the ovocytes, especially in the region that makes contact with the ovocyte/pedicel, suggesting the occurrence of changes also in the physiology of the organ. In the ovaries exposed to doses of 20 and 25 Gy, severe alterations were observed in the organ as a whole, as well as in the germ cells (oocytes) which suffered alterations in size and shape, distribution of calf granules, involvement of the DNA present in the germinal vesicle (oocyte nuclei), besides the extensive cytoplasmic vacuolization, alterations which made the maturation of these cells impossible and consequently inhibited the production of new individuals.

  • References

    1. Arthur V, Machi RA, Mastrangelo T (2015) Ionizing Radiation in Entomology: In: Topics in Ionizing Radiation Research, II Ed., In Tech. 1:1-17.
    2. Barki A, Heather N, Hendrichs J, Ferris J (2005) Fifty Years of Radiation Biology in Entomology: Lessons Learned from IDIDAS. Ann. Entomol. Soc. Am. 98:1-12.
    3. Blank, K R et al., The molecular of apoptosis and implications for radiation oncology. Int J. Radiat Biol, V. 71 , n. 5, p. 455-466, 1997.
    4. BRASIL. Ministério da Agricultura, Pecuária e Abastecimento (MAPA). DOU de 30/05/2014 (nº 102, Seção 1, pág. 55). http://www.lex.com.br/legis_25582626_Instrução Normativa N_13_DE_29_DE_MAIO_DE_2014.aspx. Acessado em 03/10/2017.
    5. Cantwell GE, Henneberry TJS (1964) The effects of gamma radiation and apholate on the reproductive tissues of Drosphila melanogaster meigen. J. Insect Pathol.: Vol: 5; Other Information: Orig. Receipt Date 1964.
    6. Camargo-Mathias MI et al. (2017) Deltamethrin as inductor agent of precocious ovarian degeneration in Rhipicephalus sanguineus s.l. (Acari: Ixodidae) ticks. Exp Appl Acarol. Springer International Publishing Switzerland.
    7. Denardi SE et al. (2004) Morphological characterization of the ovary and vitellogenesis dynamics in the tick Amblyomma cajennense (Acari: Ixodidae). Veterinary Parasitology 125:379–395.
    8. Castanha-Zanoli JC et al. (2012) Abamectin affects the bioenergetics of liver mitochondria: A potential mechanism of hepatotoxicity. Toxicol. in vit 26:51-56.
    9. El Naggar SEM, Mohamed HF, Mahmoud EA (2010) Studies on the morphology and histology of the ovary of red palm weevil female irradiated with gamma rays. Journal of Asia-Pacific Entomology 13:9–16.
    10. Hall EJ (2000) Radiobiology for the Radiologist Lippincot Williams & Wilkins,. cap. 3, cell survive curves, p. 33-50.
    11. Junqueira lC, Carneiro J (2000) Biologia Celular e Molecular. Guanabara Koogan, Rio de Janeiro, pp. 90–92.
    12. Oliveira et al. (2005) Morfological characterization of the ovary and oocytes vitelogênesis of the R. sanguineus (Acari:Ixodidae). Experimental Parasitology 110:146.
    13. Oliveira PR, Camargo-Mathias MI, Bechara GH (2006) Amblyomma triste (Koch, 1844) (Acari: Ixodidae): Morphological description of the ovary and of vitellogenesis. Experimental Parasitology 113:179–185.
    14. Oliveira PR (2010) Avaliação dos efeitos do fipronil (ingrediente ativo do FRONTLINE®) nos ovários de carrapatos Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) e no sangue periférico de roedores. Tese - Universidade Estadual Paulista, Instituto de Biociências de Rio Claro.
    15. Palma IDF (2014) Fipronil na bioenergética de mitocôndrias isoladas de figado de rato, 46 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária.
    16. Ricardo AJ et al. (2007) Ultrastructural detection of proteins, lipids and carbohydrates in oocytes of Amblyomma triste (Koch, 1844) (Acari; Ixodidae) during the vitellogenesis process. Tissue and Cell ,V.39, p. 203–215, 2007.
    17. Rocha ACP et al. (2012) Morfologia do sistema reprodutor e produção de espermatozoides de machos irradiados de Ceratitis capitata (Wied.) (Diptera: Tephritidae), linhagem tsl – Vienna 8. XXV Congresso Brasileiro de Entomologia.
    18. Rodrigues ZA (1978) Efeitos da radiação gama em Malhanarva posticata (Stal, 1855) nas condições do nordeste brasileiro. Rev. Nordest. Biol. 1:83-88.
    19. Saito KC et al. (2005) Morphological, histological, and ultrastructural studies of the ovary of the cattle- tick Boophilus microplus (Canestrini, 1887) (Acari: Ixodidae). Veterinary Parasitology n.129 p.299–31, 2005.
    20. Schimith LE et al. (2017) Exposição à permetrina no estágio larval induz disfunção mitocondrial em larvas de Danio reiro. Anais do Salão Internacional de Ensino, Pesquisa e Extensão. v.9, n.3, 2017. http://200.132.146.161/index.php/siepe/article/view/30004. Acessado em 04/01/20.
    21. SINDAN (2008) Representatividade por classe terapêutica. Sindicato Nacional da Indústria de Produtos para Saúde Animal. http://www.sindan.org.br/mercado-brasil-2018. Acessado em 17/03/2019.
    22. Silva LKF, Arthur V, Nava DE, Parra JRP (2007) Uso da radiação gama do Cobalto-60 visando ao tratamento quarentenário e a esterilização de Stenoma catenifer Walsingham (Lepidoptera: Elachistidae). Bol. San. Veg. Plagas 33:427-438.
    23. Silva LKF, Arthur V (2004) Efeito do fracionamento de dose de radiação gama sobre Sitophilus oryzae (L., 1763) (Col., Curculionidae), Rhyzopertha dominica (F., 1792) (Col., Bostrichidae) e Tribolium custanum (Herb. 1797) (Col., Tenebrionidae). Arq. lnst. Biol. 71:253-256.
    24. Sousa LAD et al. (2013) Ovary histology and quantification of hemolymph proteins of Rhipicephalus (Boophilus) microplus treated with Melia azedarach. Rev. Bras. Parasitol. Vet. 22:339-345.
    25. Walder JM, Calkins CO (1992) Gamma Radiation Effects on Ovarian Development of the Caribbean Fruit Fly, Anastrepha Suspensa (Loew) (Diptera: Tephritidae), and its Relationship to Sterile Fly Identification. Research Reports 75:2.
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How to cite

Pasqualini, A. A., Arthur, V., Schiavolin, M. B., Abreu, M. R. de, & Camargo-Mathias, M. I. (2022). Histological evaluation of <em>Rhipicephalus (Boophilus) microplus</em> tick ovaries subjected to cobalt-60 ionizing radiation. Applied Veterinary Research, 1(2), 2022007. https://doi.org/10.31893/avr.2022007
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