Susceptibility pattern in methicillin-resistant Staphylococcus aureus (MRSA) isolated from bovine mastitis

Authors

  • Mohsina Mushtaq Sher-e-Kashmir University of Agricultural Sciences and Technology, R S Pura, Jammu, Jammu and Kashmir, 180009, India https://orcid.org/0000-0002-0116-8978
  • Rajesh Agrawal Sher-e-Kashmir University of Agricultural Sciences and Technology, R S Pura, Jammu, Jammu and Kashmir, 180009, India https://orcid.org/0000-0002-8483-5923
  • Mohammad Altaf Bhat Sher-e-Kashmir University of Agricultural Sciences and Technology, R S Pura, Jammu, Jammu and Kashmir, 180009, India https://orcid.org/0000-0003-0961-4829
  • Nishi Pande Sher-e-Kashmir University of Agricultural Sciences and Technology, R S Pura, Jammu, Jammu and Kashmir, 180009, India https://orcid.org/0000-0003-1887-0875

DOI:

https://doi.org/10.31893/avr.2022003

Keywords:

antimicrobial resistance, mastitis, methicillin-resistant, Staphylococcus aureus

Abstract

The study was conducted to determine the phenotypic and genotypic antimicrobial resistance pattern of methicillin resistant Staphylococcus aureus (MRSA) isolates from bovine mastitis. A total of 160 milk samples were collected aseptically from mastitis affected cows at organized dairy farms in and around Jammu and were subjected to microbial culture for the isolation and identification of methicillin resistant S. aureus using Baird Parker Agar. Out of the total 52 Staphylococcus aureus isolates, methicillin resistance (MRSA) was recorded in only 17 (32.69%) isolates out of which 7 (41.17%) isolates carried mecA gene. Susceptibility pattern of the MRSA isolates to other antibiotics revealed highest sensitivity towards enrofloxacin (100%) and lowest towards penicillin (5.88%). Multidrug resistance (MDR) was observed in 82.35% MRSA isolates. It is concluded that enrofloxacin is the most effective drug against mastitis in terms of sensitivity but undue use of the drug must be avoided to prevent the development of resistance against this drug in the near future. Hence, accurate diagnosis of bovine mastitis along with the correct selection of antibiotic is the key to prevent this devastating disease in dairy cows. 

References

Abera M, Demie B, Aragaw K, Regassa F, Regassa A (2010) Isolation and identification of Staphylococcus aureus from bovine mastitic milk and their drug resistance patterns in Adama town, Ethiopia. Journal of Veterinary Medicine and Animal Health 2:29–34.

Baghel A, Chhabra D, Sharda R, Shukla S, Audarya S, Sikrodia R, Gangil R (2018) Isolation of Staphylococcus from bovine mastitis and their antibiotic sensitivity pattern. The Indian Journal of Veterinary Sciences and Biotechnology 13:49-52

Bauer AW, Kirby WMM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardised single disc diffusion method. American Journal of Clinical Pathology 45:493–96.

Bhat AM, Soodan JS, Singh R, Dhobi IA, Hussain T, Dar MY, Mir M (2017) Incidence of bovine clinical mastitis in Jammu region and antibiogram of isolated pathogens. Veterinary World 10:2231–916.

Chandrasekaran D, Nambi AP, Thirunavukkarasu PS, Vairamuthu S, Venkatesan P, Tirumurugaan KG (2014) A study on treatment of resistant mastitis in dairy cows. Journal of Applied Research in Clinical and Experimental Therapeutics 6:786–91.

Clinical Laboratory Standards Institute (CLSI) (2012) Performance Standards for Antimicrobial Susceptibility Testing. 15th Informational Supplement. CLSI/NCCLS document. Pennsylvania, USA.

Cuny C, Wieler LH, Wolfgang W (2015) Livestock-associated MRSA: the impact on humans. Antibiotics (Basel) 4:521-543.

Havaei SA, Assadbeigi B, Esfahani BN, Hoseini NS, Rezaei N, Havaei SR (2015) Detection of mecA and enterotoxin genes in Staphylococcus aureus isolates associated with bovine mastitis and characterization of Staphylococcal cassette chromosome mec (SCCmec) in MRSA strains. Iranian Journal of Microbiology 7:161–67.

Holmes MA, Zadoks RN (2011) Methicillin resistant S. aureus in human and bovine mastitis. Journal of Mammary Gland Biology and Neoplasia 16:373–82.

Joshi S, Ray P, Manchanda V, Bajaj J, Chitnis DS, Gautam V, Goswami P, Gupta V, Harish BN, Kagal A, Kapil A, Rao R, Rodrigues C, Sardana R, Devi KS, Sharma A, Balaji V (2013) Methicillin resistant Staphylococcus aureus (MRSA) in India: Prevalence and susceptibility pattern. Indian Journal of Medical Research 137:363–69.

Keefe G (2012) Update on control of Staphylococcus aureus and Streptococcus agalactiae for management of mastitis. Veterinary Clinics of North America: Small Animal Practice 28:203–16.

Koupahi H, Jahromy SH, Rahbar M (2016) Evaluation of different phenotypic and genotypic methods for detection of methicillin resistant Staphylococcus aureus (MRSA). Iranian Journal of Pathology 11:370–66.

Kozerski ND, Oliveira JLPD, Moura RA, Silva DRD, Oliveira AFD, Mello PL, Agostinis R O, Goncalves DD, Otutumi LK, Martins LDA (2014) Antimicrobial profile of multidrug resistant Staphylococcus spp. isolated from bovine mastitis cases in the northwest region of Parana state, Brazil. African Journal of Microbiology Research 8:3392–97.

Kumar R, Yadav BR, Singh RS (2011) Antibiotic resistance and pathogenicity factors in Staphylococcus aureus isolated from mastitic Sahiwal cattle. Journal of Biological Sciences 36:175–88.

Kuyma K, Malinowski E, Lassa H, Kossowska A (2003) Specific detection of Staphylococcus aureus by PCR in intramammary infection. Bulletin of the Veterinary Institute in Pulawy 47:183–90.

Lee JH (2003) Methicillin (oxacillin) resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Applied and Environmental Microbiology 69:6489-6494.

Mehndiratta PL, Bhalla P (2012) Typing of Methicillin resistant Staphylococcus aureus: a technical review. Indian Journal of Medical Microbiology 30:16-23.

Olayinka BO, Olayinka AT, Obajuluwa AF, Onaolapo JA, Olurinola PF (2009) Absence of mecA gene in methicillin-resistant Staphylococcus aureus isolates. African Journal of Infectious Diseases 3:49-56.

Paterson GK, Harrison EM, Holmes MA (2014) The emergence of mecC methicillin-resistant Staphylococcus aureus. Trends in Microbiology 22:42–47.

Radostits OM, Gay CC, Hinchcliff KW, Constable PD (2007) Veterinary Medicine: A text book of the disease of cattle, horses, sheep, pigs and goats. 10th ed. London: Elsevier Ltd.

Ruegg PL (2017) A 100-year review: mastitis detection, management, and prevention. Journal of Dairy Science 100:10381–10397.

Sancak B (2000) S. aureus dametisilindirençmekanizmalari. Mikrobiyoloji Bulteni 34:381–89.

Sharma A, Sindhu N (2007) Occurrence of clinical and subclinical mastitis in buffaloes in the state of Haryana (India). Italian Journal of Animal Science 6:965-967.

Umaru GA, Kwaga JKP, Bello M, Raji MA, Maitala YS (2016) Antibiotic resistance of Staphylococcus aureus isolated from fresh cow milk in settled Fulani herds in Kaduna state, Nigeria. Bulletin of Animal Health and Production in Africa 64:173–82.

Zaatout N, Ayachi A, Kecha, Kaldec K (2019) Identification of Staphylococci causing mastitis in dairy cattle from Algeria and characterization of Staphylococcus aureus. Journal of Applied Microbiology 127:1305-1314.

PCR amplification of nuc gene at 270 bp. Lane M - 100bp DNA ladder; Lane P - Positive control; Lane N - Negative control; Lane 1-7 - Positive isolates

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CITATION

PUBLISHED: 22-01-2022

How to Cite

Mushtaq, M., Agrawal, R., Bhat, M. A. ., & Pande, N. . (2022). Susceptibility pattern in methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) isolated from bovine mastitis. Applied Veterinary Research, 1(1), e2022003. https://doi.org/10.31893/avr.2022003

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Research Article