Susceptibility pattern in methicillin-resistant Staphylococcus aureus (MRSA) isolated from bovine mastitis
Keywords:antimicrobial resistance, mastitis, methicillin-resistant, Staphylococcus aureus
The study was conducted to determine the phenotypic and genotypic antimicrobial resistance pattern of methicillin resistant Staphylococcus aureus (MRSA) isolates from bovine mastitis. A total of 160 milk samples were collected aseptically from mastitis affected cows at organized dairy farms in and around Jammu and were subjected to microbial culture for the isolation and identification of methicillin resistant S. aureus using Baird Parker Agar. Out of the total 52 Staphylococcus aureus isolates, methicillin resistance (MRSA) was recorded in only 17 (32.69%) isolates out of which 7 (41.17%) isolates carried mecA gene. Susceptibility pattern of the MRSA isolates to other antibiotics revealed highest sensitivity towards enrofloxacin (100%) and lowest towards penicillin (5.88%). Multidrug resistance (MDR) was observed in 82.35% MRSA isolates. It is concluded that enrofloxacin is the most effective drug against mastitis in terms of sensitivity but undue use of the drug must be avoided to prevent the development of resistance against this drug in the near future. Hence, accurate diagnosis of bovine mastitis along with the correct selection of antibiotic is the key to prevent this devastating disease in dairy cows.
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